ArticleJournal of industrial microbiology & biotechnology2018
Engineering co-culture system for production of apigetrin in Escherichia coli.
Article in Journal of industrial microbiology & biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 59 citations in OpenAlex.
- Cooperativity in microbial biotechnology: synthetic consortia as emerging metabolic engineering strategy for sustainable bioproduction.Frontiers in bioengineering and biotechnology · 2026Review
- Strategies and tools to construct stable and efficient artificial coculture systems as biosynthetic platforms for biomass conversion.Biotechnology for biofuels and bioproducts · 2024Review
- Biosynthesis of Apigenin Glucosides in EngineeredJournal of microbiology and biotechnology · 2024Article
- Efficient Biosynthesis of Salidroside via ArtificialACS omega · 2024Article
- Metabolic engineering and optimization of Escherichia coli co-culture for the de novo synthesis of genkwanin.Journal of industrial microbiology & biotechnology · 2023Article
- Flavonoid Production: Current Trends in Plant Metabolic Engineering and De Novo Microbial Production.Metabolites · 2023Review
- Artificial microbial consortia for bioproduction processes.Engineering in life sciences · 2023Review
- Article
- Apigetrin Abrogates Lipopolysaccharide-Induced Inflammation in L6 Skeletal Muscle Cells through NF-κB/MAPK Signaling Pathways.Current issues in molecular biology · 2022Article
- Bioproduction of eriodictyol by Escherichia coli engineered co-culture.World journal of microbiology & biotechnology · 2022Article
- Review
- Methods for the Development of Recombinant Microorganisms for the Production of Natural Products.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Engineered biosynthesis of plant polyketides by type III polyketide synthases in microorganisms.Frontiers in bioengineering and biotechnology · 2022Review
- Metabolic Engineering of Microbial Cell Factories for Biosynthesis of Flavonoids: A Review.Molecules (Basel, Switzerland) · 2021Review
- Native and non-native host assessment towards metabolic pathway reconstructions of plant natural products.Biotechnology reports (Amsterdam, Netherlands) · 2021Review
- Two-species community design of lactic acid bacteria for optimal production of lactate.Computational and structural biotechnology journal · 2021Article
- Metabolic Engineering of Microorganisms for the Production of Flavonoids.Frontiers in bioengineering and biotechnology · 2020Review
- Advances in the development and application of microbial consortia for metabolic engineering.Metabolic engineering communications · 2019Review
- Emerging strategies for engineering microbial communities.Biotechnology advances · 2019Review
- Escherichia coli modular coculture system for resveratrol glucosides production.World journal of microbiology & biotechnology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 2 countries.
Funding
Abstract
Microbial cells have extensively been utilized to produce value-added bioactive compounds. Based on advancement in protein engineering, DNA recombinant technology, genome engineering, and metabolic remodeling, the microbes can be re-engineered to produce industrially and medicinally important platform chemicals. The emergence of co-culture system which reduces the metabolic burden and allows parallel optimization of the engineered pathway in a modular fashion restricting the formation of undesired byproducts has become an alternative way to synthesize and produce bioactive compounds. In this study, we present genetically engineered E. coli-based co-culture system to the de novo synthesis of apigetrin (APG), an apigenin-7-O-β-D-glucopyranoside of apigenin. The culture system consists of an upstream module including 4-coumarate: CoA ligase (4CL), chalcone synthase, chalcone flavanone isomerase (CHS, CHI), and flavone synthase I (FNSI) to synthesize apigenin (API) from p-coumaric acid (PCA). Whereas, the downstream system contains a metabolizing module to enhance the production of UDP-glucose and expression of glycosyltransferase (PaGT3) to convert API into APG. To accomplish this improvement in titer, the initial inoculum ratio of strains for making the co-culture system, temperature, and media component was optimized. Following large-scale production, a yield of 38.5 µM (16.6 mg/L) of APG was achieved. In overall, this study provided an efficient tool to synthesize bioactive compounds in microbial cells.
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Registered trials
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