ArticleMarine biotechnology (New York, N.Y.)2018
Cloning, Expression, and Biochemical Characterization of Two New Oligoalginate Lyases with Synergistic Degradation Capability.
Article in Marine biotechnology (New York, N.Y.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 45 citations in OpenAlex.
- Enhanced Thermostability and Catalytic Efficiency of Alginate Lyase Alyw203 by Hydrogen Bond Network Reconstruction.Marine drugs · 2025Article
- Genome Analysis of a Potential NovelMarine drugs · 2024Article
- Three alginate lyases provide a new gutApplied and environmental microbiology · 2023Article
- Characterization of degradation patterns and enzymatic properties of a novel alkali-resistant alginate lyase AlyRm1 fromCurrent research in food science · 2023Article
- Degradation of Alginate by a Newly Isolated Marine BacteriumMarine drugs · 2022Article
- Cloning and Characterization of a Novel Alginate Lyase fromMarine drugs · 2022Article
- An efficient enzyme-triggered controlled release system for colon-targeted oral delivery to combat dextran sodium sulfate (DSS)-induced colitis in mice.Drug delivery · 2021Article
- Expression and Characterization of a Cold-Adapted Alginate Lyase with Exo/Endo-Type Activity from a Novel Marine BacteriumMarine drugs · 2021Article
- Article
- Biochemical characteristics and molecular mechanism of an exo-type alginate lyase VxAly7D and its use for the preparation of unsaturated monosaccharides.Biotechnology for biofuels · 2020Article
- Characterization of a bifunctional alginate lyase as a new member of the polysaccharide lyase family 17 from a marine strain BP-2.Biotechnology letters · 2019Article
- Efficiently Anti-Obesity Effects of Unsaturated Alginate Oligosaccharides (UAOS) in High-Fat Diet (HFD)-Fed Mice.Marine drugs · 2019Article
- Rational Design of Alginate Lyase fromMarine drugs · 2019Article
- Biochemical Characterization and Elucidation of Action Pattern of a Novel Polysaccharide Lyase 6 Family Alginate Lyase from Marine BacteriumMarine drugs · 2019Article
- Biochemical characteristics and synergistic effect of two novel alginate lyases fromBiotechnology for biofuels · 2019Article
- Expression and Characterization of a New PolyG-Specific Alginate Lyase From Marine BacteriumFrontiers in microbiology · 2018Article
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6 authors at 2 institutions in 1 country.
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Abstract
Alginate, the most abundant carbohydrate presents in brown macroalgae, has recently gained increasing attention as an alternative biomass for the production of biofuel. Oligoalginate lyases catalyze the degradation of alginate oligomers into monomers, a prerequisite for bioethanol production. In this study, two new oligoalginate lyase genes, oalC6 and oalC17, were cloned from Cellulophaga sp. SY116, and expressed them in Escherichia coli. The deduced oligoalginate lyases, OalC6 and OalC17, belonged to the polysaccharide lyase (PL) family 6 and 17, respectively. Both showed less than 50% amino acid identity with all of the characterized oligoalginate lyases. Moreover, OalC6 and OalC17 could degrade both alginate polymers and oligomers into monomers in an exolytic mode. Substrate specificity studies demonstrated that OalC6 preferred α-L-guluronate (polyG) blocks, while OalC17 preferred poly β-D-mannuronate (polyM) blocks. The combination of OalC6 and OalC17 showed synergistic degradation ability toward both alginate polymers and oligomers. Finally, an efficient process for the production of alginate monomers was established by combining the new-isolated exotype alginate lyases (i.e., OalC6 and OalC17) and the endotype alginate lyase AlySY08. Overall, our work provides new insights for the development of novel biotechnologies for biofuel production from seaweed.
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