ArticleScientific reports2018
Whole Organ Blood and Lymphatic Vessels Imaging (WOBLI).
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Slide-free FIBI histology for improved quantitative vascular network analysis in breast cancer: Integrating vessel geometry and topology metrics.Journal of pathology informatics · 2026Article
- 3D visualization of uterus and ovary: tissue clearing techniques and biomedical applications.Frontiers in bioengineering and biotechnology · 2025Review
- Deep learning-based vessel extraction in 3D confocal microscope images of cleared human glioma tissues.Biomedical optics express · 2024Article
- Semi-automated protocol to quantify and characterize fluorescent three-dimensional vascular images.PloS one · 2024Article
- 3D imaging and morphometric descriptors of vascular networks on optically cleared organs.iScience · 2023Review
- Visualization of Organ-Specific Lymphatic Growth: An Efficient Approach to Labeling Molecular Markers in Cleared Tissues.International journal of molecular sciences · 2023Article
- Tissue clearing and immunostaining to visualize the spatial organization of vasculature and tumor cells in mouse liver.Frontiers in oncology · 2023Article
- An analysis modality for vascular structures combining tissue-clearing technology and topological data analysis.Nature communications · 2022Article
- Optical clearing and testing of lung tissue using inhalation aerosols: prospects for monitoring the action of viral infections.Biophysical reviews · 2022Review
- Hierarchical imaging and computational analysis of three-dimensional vascular network architecture in the entire postnatal and adult mouse brain.Nature protocols · 2021Review
- Ovary Development: Insights From a Three-Dimensional Imaging Revolution.Frontiers in cell and developmental biology · 2021Review
- BACH family members regulate angiogenesis and lymphangiogenesis by modulating VEGFC expression.Life science alliance · 2020Article
- Three-Dimensional Micro-Computed Tomography of the Adult Mouse Ovary.Frontiers in cell and developmental biology · 2020Article
- 3-dimensional visualization of implant-tissue interface with the polyethylene glycol associated solvent system tissue clearing method.Cell proliferation · 2019Article
- Imaging of Murine Whole Lung Fibrosis by Large Scale 3D Microscopy aided by Tissue Optical Clearing.Scientific reports · 2018Article
- Spatial and temporal changes in extracellular elastin and laminin distribution during lung alveolar development.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Thin section histology is limited in providing 3D structural information, particularly of the intricate morphology of the vasculature. Availability of high spatial resolution imaging for thick samples, would overcome the restriction dictated by low light penetration. Our study aimed at optimizing the procedure for efficient and affordable tissue clearing, along with an appropriate immunofluorescence labeling that will be applicable for high resolution imaging of blood and lymphatic vessels. The new procedure, termed whole organ blood and lymphatic vessels imaging (WOBLI), is based on two previously reported methods, CLARITY and ScaleA2. We used this procedure for the analysis of isolated whole ovary, uterus, lung and liver. These organs were subjected to passive clearing, following fixation, immunolabeling and embedding in hydrogel. Cleared specimens were immersed in ScaleA2 solution until transparency was achieved and imaged using light sheet microscopy. We demonstrate that WOBLI allows detailed analysis and generation of structural information of the lymphatic and blood vasculature from thick slices and more importantly, from whole organs. We conclude that WOBLI offers the advantages of morphology and fluorescence preservation with efficient clearing. Furthermore, WOBLI provides a robust, cost-effective method for generation of transparent specimens, allowing high resolution, 3D-imaging of blood and lymphatic vessels networks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.