ArticleScientific reports2018
3D virtual histology of murine kidneys -high resolution visualization of pathological alterations by micro computed tomography.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 60 citations in OpenAlex.
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- Nephron number variability in Japanese subjects: an autopsy-based study and its implications for chronic kidney disease: clinical scientist award address 2024.Clinical and experimental nephrology · 2025Review
- Intravital imaging of peritubular microcirculation impairment in cisplatin-induced acute kidney injury.JCI insight · 2025Article
- Characterizing Metabolic Shifts in Septic Murine Kidney Tissue Using 2P-FLIM for Early Sepsis Detection.Bioengineering (Basel, Switzerland) · 2025Article
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- Three-dimensional virtual histology of the rat uterus musculature using micro-computed tomography.Journal of anatomy · 2025Article
- The SmARTR pipeline: A modular workflow for the cinematic rendering of 3D scientific imaging data.iScience · 2024Article
- Three-Dimensional Cell Culture Micro-CT Visualization within Collagen Scaffolds in an Aqueous Environment.Cells · 2024Article
- X-Ray-Based 3D Histopathology of the Kidney Using Cryogenic Contrast-Enhanced MicroCT.International journal of biomedical imaging · 2024Article
- Engineering the future of 3D pathology.The journal of pathology. Clinical research · 2024Article
- Optimal Agents for Visualizing Collagen Tissue Microarchitecture Using Contrast-Enhanced MicroCT.Pharmaceuticals (Basel, Switzerland) · 2023Review
- A hybrid approach to full-scale reconstruction of renal arterial network.Scientific reports · 2023Article
- Three-dimensional analysis of human pancreatic cancer specimens by phase-contrast based X-ray tomography - the next dimension of diagnosis.Cancer imaging : the official publication of the International Cancer Imaging Society · 2023Article
- Analysis of RNA processing directly from spatial transcriptomics data reveals previously unknown regulation.bioRxiv : the preprint server for biology · 2023Article
- Phosphotungstic acid-enhanced microcomputed tomography for quantitative visualization of mouse mammary gland morphology.Journal of medical imaging (Bellingham, Wash.) · 2023Article
- Semibulk RNA-seq analysis as a convenient method for measuring gene expression statuses in a local cellular environment.Scientific reports · 2022Article
- Pericyte-mediated constriction of renal capillaries evokes no-reflow and kidney injury following ischaemia.eLife · 2022Article
- Brain virtual histology with X-ray phase-contrast tomography Part I: whole-brain myelin mapping in white-matter injury models.Biomedical optics express · 2022Article
- Three-dimensional reconstruction of testis cords/seminiferous tubules.Reproductive medicine and biology · 2021Review
- X-ray Dark-Field Imaging (XDFI)-a Promising Tool for 3D Virtual Histopathology.Molecular imaging and biology · 2021Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The increasing number of patients with end stage chronic kidney disease not only calls for novel therapeutics but also for pioneering research using convincing preclinical disease models and innovative analytical techniques. The aim of this study was to introduce a virtual histology approach using micro computed tomography (µCT) for the entire murine kidney in order to close the gap between single slice planar histology and a 3D high resolution dataset. An ex vivo staining protocol based on phosphotungstic acid diffusion was adapted to enhance renal soft tissue x-ray attenuation. Subsequent CT scans allowed (i) the detection of the renal cortex, medulla and pelvis in greater detail, (ii) the analysis of morphological alterations, (iii) the quantification of the volume as well as the radio-opacity of these portions and (iv) the quantification of renal fibrotic remodeling based on altered radio-opacity using the unilateral ureteral obstruction model. Thus, virtual histology based on PTA contrast enhanced CT will in future help to refine the outcome of preclinical research on kidney associated murine disease models.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.