ArticleScientific reports2018
Low cost, microcontroller based heating device for multi-wavelength differential scanning fluorimetry.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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Who cites it
6 citing papers in PubMed.
- Structure of the turnover-ready state of an ancestral respiratory complex I.Nature communications · 2024Article
- Membrane-domain mutations in respiratory complex I impede catalysis but do not uncouple proton pumping from ubiquinone reduction.PNAS nexus · 2022Article
- A low-cost 3D-printable differential scanning fluorometer for protein and RNA melting experiments.HardwareX · 2022Article
- Mutations in respiratory complex I promote antibiotic persistence through alterations in intracellular acidity and protein synthesis.Nature communications · 2022Article
- A mechanism to prevent production of reactive oxygen species by Escherichia coli respiratory complex I.Nature communications · 2019Article
- Isothermal Analysis of ThermoFluor Data can readily provide Quantitative Binding Affinities.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Differential scanning fluorimetry is a popular method to estimate the stability of a protein in distinct buffer conditions by determining its 'melting point'. The method requires a temperature controlled fluorescence spectrometer or a RT-PCR machine. Here, we introduce a low-budget version of a microcontroller based heating device implemented into a 96-well plate reader that is connected to a standard fluorescence spectrometer. We demonstrate its potential to determine the 'melting point' of soluble and membranous proteins at various buffer conditions.
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Registered trials
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