Evidence map›Paper›PMID 29361546›Full record

ArticleJournal of cell science2018

A 'molecular guillotine' reveals the interphase function of Kinesin-5.

Zhiyi Lv, Jan Rosenbaum, Timo Aspelmeier, Jörg Großhans

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cell science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.1field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Zhiyi LvInstitute for Developmental Biochemistry, Medical School, University of Göttingen, Justus-von-Liebig Weg 11, 37077 Göttingen, Germany.
Jan RosenbaumInstitute for Mathematical Stochastics and Felix Bernstein Institute for Mathematical Statistics in the Biosciences, University of Göttingen, Goldschmidtstraße 7, 37077 Göttingen, Germany.
Timo AspelmeierInstitute for Mathematical Stochastics and Felix Bernstein Institute for Mathematical Statistics in the Biosciences, University of Göttingen, Goldschmidtstraße 7, 37077 Göttingen, Germany.
Jörg GroßhansInstitute for Developmental Biochemistry, Medical School, University of Göttingen, Justus-von-Liebig Weg 11, 37077 Göttingen, Germany jgrossh@gwdg.de.ORCID 0000-0003-1114-9233
University of Göttingen · DE

Funding

Resource Component: Acquisition, maintenance and distribution of Drosophila stocksP40OD018537 · OD · TRUSTEES OF INDIANA UNIVERSITY · PI Annette L. Parks · 2014 to 2026
$13.5M
DROSOPHILA GENOMICS RESOURCE CENTERP40OD010949 · OD · TRUSTEES OF INDIANA UNIVERSITY · PI Andrew Zelhof · 2012 to 2026
$9.4M
NIH HHS P40 OD010949NIH HHS P40 OD018537
6 · The paper itself

Abstract

Motor proteins are important for transport and force generation in a variety of cellular processes and in morphogenesis. Here, we describe a general strategy for conditional motor mutants by inserting a protease cleavage site into the 'neck' between the head domain and the stalk of the motor protein, making the protein susceptible to proteolytic cleavage at the neck by the corresponding protease. To demonstrate the feasibility of this approach, we inserted the cleavage site of the tobacco etch virus (TEV) protease into the neck of the tetrameric motor Kinesin-5. Application of TEV protease led to a specific depletion and functional loss of Kinesin-5 in

Indexed as

InterphaseAmino Acid SequenceAnimalsCentrosomeDrosophila melanogasterDrosophila ProteinsEmbryo, NonmammalianEndopeptidasesGreen Fluorescent ProteinsMicrotubule-Associated ProteinsMitosisPhenotypeDrosophila ProteinsEndopeptidasesGreen Fluorescent ProteinsKlp61F protein, DrosophilaMicrotubule-Associated ProteinsTEV proteaseCentrosomesConditional mutantDrosophilaFluctuation analysisKinesinMolecular motorProtease

Identifiers

PMID29361546
PMCPMC5826049
OpenAlexW2751556519

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.