ArticleJournal of experimental botany2018
Scopoletin 8-hydroxylase: a novel enzyme involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis.
Article in Journal of experimental botany, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 140 citations in OpenAlex.
- A bHLH121/URI-ANAC017-MAT1/SAM1 regulatory cascade controls iron homeostasis in Arabidopsis.The Plant journal : for cell and molecular biology · 2026Article
- Sarcandra glabra genome unveils functional conservation and divergence of O-methyltransferases catalyzing isofraxidin biosynthesis in angiosperms.Nature communications · 2026Article
- Simultaneous Overexpression of FERULOYL-CoA 6'-HYDROXYLASE 1 and COUMARIN SYNTHASE Leads to Coumarin-Enriched Lignin and Improved Saccharification in Greenhouse- and Field-Grown Poplar.Plant biotechnology journal · 2026Article
- Coumarin Reduces Aluminum-Induced Inhibition of Growth and Photosynthesis inPlants (Basel, Switzerland) · 2026Article
- A Substrate-Dependent Bifunctional Dioxygenase fromMolecules (Basel, Switzerland) · 2026Article
- The Arabidopsis NPF7.2 mediates coumarin uptake for root iron acquisition.The New phytologist · 2026Article
- Anti-cancer Potential of Scopoletin Derivatives Across Diverse Cancer Cell Lines: A Comprehensive Review.Current drug discovery technologies · 2026Review
- Cooperation between a root fungal endophyte and host-derived coumarin scopoletin mediates Arabidopsis iron nutrition.The New phytologist · 2025Article
- Coumarin-facilitated iron transport: An IRT1-independent strategy for iron acquisition in Arabidopsis thaliana.Plant communications · 2025Article
- Chemical Profiling and UPLC-qTOF-MS/MS-Based Metabolomics of Three Different Parts ofNutrients · 2025Article
- Engineering yeast to produce fraxetin from ferulic acid and lignin.Applied microbiology and biotechnology · 2025Article
- Medicago truncatula genotype drives the plant nutritional strategy and its associated rhizosphere bacterial communities.The New phytologist · 2025Article
- The biosynthetic pathway of coumarin and its genetic regulation in response to biotic and abiotic stresses.Frontiers in plant science · 2025Review
- The Ameliorative Effect of Coumarin on Copper Toxicity inPlants (Basel, Switzerland) · 2024Article
- RNA-Seq and WGCNA Analyses Reveal Key Regulatory Modules and Genes for Salt Tolerance in Cotton.Genes · 2024Article
- Genes to specialized metabolites: accumulation of scopoletin, umbelliferone and their glycosides in natural populations of Arabidopsis thaliana.BMC plant biology · 2024Article
- The gradual establishment of complex coumarin biosynthetic pathway in Apiaceae.Nature communications · 2024Article
- A major role of coumarin-dependent ferric iron reduction in strategy I-type iron acquisition in Arabidopsis.The Plant cell · 2024Article
- Stability of Coumarins and Determination of the Net Iron Oxidation State of Iron-Coumarin Complexes: Implications for Examining Plant Iron Acquisition Mechanisms.ACS earth & space chemistry · 2023Article
- Engineered coumarin accumulation reduces mycotoxin-induced oxidative stress and disease susceptibility.Plant biotechnology journal · 2023Article
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Iron deficiency is a serious agricultural problem, particularly in alkaline soils. Secretion of coumarins by Arabidopsis thaliana roots is induced under iron deficiency. An essential enzyme for the biosynthesis of the major Arabidopsis coumarins, scopoletin and its derivatives, is Feruloyl-CoA 6'-Hydroxylase1 (F6'H1), which belongs to a large enzyme family of the 2-oxoglutarate and Fe2+-dependent dioxygenases. We have functionally characterized another enzyme of this family, which is a close homologue of F6'H1 and is encoded by a strongly iron-responsive gene, At3g12900. We purified At3g12900 protein heterologously expressed in Escherichia coli and demonstrated that it is involved in the conversion of scopoletin into fraxetin, via hydroxylation at the C8 position, and that it thus functions as a scopoletin 8-hydroxylase (S8H). Its function in plant cells was confirmed by the transient expression of S8H protein in Nicotiana benthamiana leaves, followed by metabolite profiling and biochemical and ionomic characterization of Arabidopsis s8h knockout lines grown under various iron regimes. Our results indicate that S8H is involved in coumarin biosynthesis, as part of mechanisms used by plants to assimilate iron.
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