ArticleNucleic acids research2018
A sigma factor toolbox for orthogonal gene expression in Escherichia coli.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- Mixed positive and negative feedback loops drive diverse single-cell gene expression dynamics.Nature communications · 2026Article
- Synthetic Biology Strategies for Activating Cryptic BGCs inACS omega · 2026Review
- Computation-guided redesign of promoter specificity of a bacterial RNA polymerase.bioRxiv : the preprint server for biology · 2025Article
- Physiological roles of anmBio · 2025Article
- Expanding the σ54-dependent transcription process with orthogonal designs.Nucleic acids research · 2025Article
- Negative dataset selection impacts machine learning-based predictors for multiple bacterial species promoters.Bioinformatics (Oxford, England) · 2025Article
- Biosensor characterization: formal methods from the perspective of proteome fractions.Synthetic biology (Oxford, England) · 2025Article
- Engineered Phage Enables Efficient Control of Gene Expression upon Infection of the Host Cell.International journal of molecular sciences · 2024Article
- Assembly of functional microbial ecosystems: from molecular circuits to communities.FEMS microbiology reviews · 2024Review
- Generating information-dense promoter sequences with optimal string packing.PLoS computational biology · 2024Article
- Towards a rational approach to promoter engineering: understanding the complexity of transcription initiation in prokaryotes.FEMS microbiology reviews · 2024Review
- A MoClo-Compatible Toolbox of ECF Sigma Factor-Based Regulatory Switches for Proteobacterial Chassis.Biodesign research · 2024Article
- Deep Learning-Assisted Design of Novel Promoters inAdvanced genetics (Hoboken, N.J.) · 2023Article
- Dissecting the Determinants of Domain Insertion Tolerance and Allostery in Proteins.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- The σB alternative sigma factor circuit modulates noise to generate different types of pulsing dynamics.PLoS computational biology · 2023Article
- Regulation strategies for two-output biomolecular networks.Journal of the Royal Society, Interface · 2023Article
- Understanding Autologous Spliceostatin Transcriptional Regulation to Derive Parts for Heterologous Expression in aACS synthetic biology · 2023Article
- Using CIVT-SELEX to Select Aptamers as Genetic Parts to Regulate Gene Circuits in a Cell-Free System.International journal of molecular sciences · 2023Article
- Dynamic feedback regulation for efficient membrane protein production using a small RNA-based genetic circuit in Escherichia coli.Microbial cell factories · 2022Article
- Plasmids for Controlled and Tunable High-Level Expression in E. coli.Applied and environmental microbiology · 2022Article
Corrections and comments
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Synthetic genetic sensors and circuits enable programmable control over timing and conditions of gene expression and, as a result, are increasingly incorporated into the control of complex and multi-gene pathways. Size and complexity of genetic circuits are growing, but stay limited by a shortage of regulatory parts that can be used without interference. Therefore, orthogonal expression and regulation systems are needed to minimize undesired crosstalk and allow for dynamic control of separate modules. This work presents a set of orthogonal expression systems for use in Escherichia coli based on heterologous sigma factors from Bacillus subtilis that recognize specific promoter sequences. Up to four of the analyzed sigma factors can be combined to function orthogonally between each other and toward the host. Additionally, the toolbox is expanded by creating promoter libraries for three sigma factors without loss of their orthogonal nature. As this set covers a wide range of transcription initiation frequencies, it enables tuning of multiple outputs of the circuit in response to different sensory signals in an orthogonal manner. This sigma factor toolbox constitutes an interesting expansion of the synthetic biology toolbox and may contribute to the assembly of more complex synthetic genetic systems in the future.
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