ArticleNucleic acids research2018
In vitro isolation of small-molecule-binding aptamers with intrinsic dye-displacement functionality.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 48 citations in OpenAlex.
- Article
- Developing Aptamer-Based Colorimetric Opioid Tests.JACS Au · 2024Article
- Rationally Designed DNA-Based Scaffolds and Switching Probes for Protein Sensing.Advances in biochemical engineering/biotechnology · 2024Review
- High-throughput quantitative binding analysis of DNA aptamers using exonucleases.Nucleic acids research · 2023Article
- Capture-SELEX: Selection Strategy, Aptamer Identification, and Biosensing Application.Biosensors · 2022Review
- RT-qPCR as a screening platform for mutational and small molecule impacts on structural stability of RNA tertiary structures.RSC chemical biology · 2022Article
- Combating small molecule environmental contaminants: detection and sequestration using functional nucleic acids.Chemical science · 2022Review
- Uptake mechanisms of cell-internalizing nucleic acid aptamers for applications as pharmacological agents.RSC medicinal chemistry · 2021Review
- Recent Advances in Micro/Nanomaterial-Based Aptamer Selection Strategies.Molecules (Basel, Switzerland) · 2021Review
- Advances and Challenges in Small-Molecule DNA Aptamer Isolation, Characterization, and Sensor Development.Angewandte Chemie (International ed. in English) · 2021Review
- Isolation of Natural DNA Aptamers for Challenging Small-Molecule Targets, Cannabinoids.Analytical chemistry · 2021Article
- Label-free profiling of DNA aptamer-small molecule binding using T5 exonuclease.Nucleic acids research · 2020Article
- GOLD SELEX: a novel SELEX approach for the development of high-affinity aptamers against small molecules without residual activity.Mikrochimica acta · 2020Article
- Perspective on the Future Role of Aptamers in Analytical Chemistry.Analytical chemistry · 2019Article
- Innovative engineering and sensing strategies for aptamer-based small-molecule detection.Trends in analytical chemistry : TRAC · 2019Article
- In vitro isolation of class-specific oligonucleotide-based small-molecule receptors.Nucleic acids research · 2019Article
- Label-Free, Visual Detection of Small Molecules Using Highly Target-Responsive Multimodule Split Aptamer Constructs.Analytical chemistry · 2019Article
- Updates on Aptamer Research.International journal of molecular sciences · 2019Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Aptamer-based sensors offer a powerful tool for molecular detection, but the practical implementation of these biosensors is hindered by costly and laborious sequence engineering and chemical modification procedures. We report a simple strategy for directly isolating signal-reporting aptamers in vitro through systematic evolution of ligands by exponential enrichment (SELEX) that transduce binding events into a detectable change of absorbance via target-induced displacement of a small-molecule dye. We first demonstrate that diethylthiatricarbocyanine (Cy7) can stack into DNA three-way junctions (TWJs) in a sequence-independent fashion, greatly altering the dye's absorbance spectrum. We then design a TWJ-containing structured library and isolate an aptamer against 3,4-methylenedioxypyrovalerone (MDPV), a synthetic cathinone that is an emerging drug of abuse. This aptamer intrinsically binds Cy7 within its TWJ domain, but MDPV efficiently displaces the dye, resulting in a change in absorbance within seconds. This assay is label-free, and detects nanomolar concentrations of MDPV. It also recognizes other synthetic cathinones, offering the potential to detect newly-emerging designer drugs, but does not detect structurally-similar non-cathinone compounds or common cutting agents. Moreover, we demonstrate that the Cy7-displacement colorimetric assay is more sensitive than a conventional strand-displacement fluorescence assay. We believe our strategy offers an effective generalized approach for the development of sensitive dye-displacement colorimetric assays for other small-molecule targets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.