ArticleNucleic acids research2018
Selective terminal methylation of a tRNA wobble base.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- RNA modification-mediated multidimensional crosstalk: a novel perspective on gene expression regulation.Genome biology · 2026Review
- Emerging roles of tRNA modification-mediated codon-specific translational reprogramming in cancer biology.Cell death & disease · 2026Review
- tRNA-modifying enzymes in bacterial stress adaptation.Open biology · 2025Review
- An RNA modification prevents extended codon-anticodon interactions from facilitating +1 frameshifting.Nature communications · 2025Article
- RNA modifying enzymes shape tRNA biogenesis and function.The Journal of biological chemistry · 2024Review
- A tRNA modification pattern that facilitates interpretation of the genetic code.Frontiers in microbiology · 2024Article
- tRNA methylation resolves codon usage bias at the limit of cell viability.Cell reports · 2022Article
- Genome Expansion by tRNA +1 Frameshifting at Quadruplet Codons.Journal of molecular biology · 2022Review
- Twice exploration of tRNA +1 frameshifting in an elongation cycle of protein synthesis.Nucleic acids research · 2021Article
- The occurrence order and cross-talk of different tRNA modifications.Science China. Life sciences · 2021Review
- Article
- tRNA elbow modifications affect the tRNA pseudouridine synthase TruB and the methyltransferase TrmA.RNA (New York, N.Y.) · 2020Article
- Intellectual disability-associated gene ftsj1 is responsible for 2'-O-methylation of specific tRNAs.EMBO reports · 2020Article
- tRNA Methylation Is a Global Determinant of Bacterial Multi-drug Resistance.Cell systems · 2019Article
- tRNA 3'-amino-tailing for stable amino acid attachment.RNA (New York, N.Y.) · 2018Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Active tRNAs are extensively post-transcriptionally modified, particularly at the wobble position 34 and the position 37 on the 3'-side of the anticodon. The 5-carboxy-methoxy modification of U34 (cmo5U34) is present in Gram-negative tRNAs for six amino acids (Ala, Ser, Pro, Thr, Leu and Val), four of which (Ala, Ser, Pro and Thr) have a terminal methyl group to form 5-methoxy-carbonyl-methoxy-uridine (mcmo5U34) for higher reading-frame accuracy. The molecular basis for the selective terminal methylation is not understood. Many cmo5U34-tRNAs are essential for growth and cannot be substituted for mutational analysis. We show here that, with a novel genetic approach, we have created and isolated mutants of Escherichia coli tRNAPro and tRNAVal for analysis of the selective terminal methylation. We show that substitution of G35 in the anticodon of tRNAPro inactivates the terminal methylation, whereas introduction of G35 to tRNAVal confers it, indicating that G35 is a major determinant for the selectivity. We also show that, in tRNAPro, the terminal methylation at U34 is dependent on the primary m1G methylation at position 37 but not vice versa, indicating a hierarchical ranking of modifications between positions 34 and 37. We suggest that this hierarchy provides a mechanism to ensure top performance of a tRNA inside of cells.
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