ArticlePloS one2018
Effects of dietary supplementation of arginine-silicate-inositol complex on absorption and metabolism of calcium of laying hens.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- Dietary Arginine Supplementation Modulates Nitrogen Utilization, Serum Biochemistry, and Gut Microbiota in Growing Beagles Under Low- and Normal-Protein Diets.Animals : an open access journal from MDPI · 2026Article
- The Beneficial Effects of Guanidinoacetic Acid as a Functional Feed Additive: A Possible Approach for Poultry Production.Veterinary sciences · 2026Review
- Regulatory effects of hawthorn leaf flavonoids and stevioside on the uterine function and eggshell quality in laying hens.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Article
- Dietary glycerol monolaurate mitigates heat stress-induced disruption of intestinal homeostasis and hepatic lipid metabolism in laying hens.Stress biology · 2025Article
- Effects of Apilarnil on Type 2 Diabetes-Induced IRS-1/PI3K/Akt Mediated Insulin Resistance in Male Rats.ACS omega · 2025Article
- Influence of Silicon Supplementation on Growth, Immunity, Antioxidant, Hormonal Profile and Bone Health Biomarkers in Pre-ruminant Crossbred Calves.Biological trace element research · 2025Article
- Effects of Dietary Supplementation of Arginine-Silicate- Inositol and Phytase Complex on Performance and Blood Biochemical Traits of Laying Hens.Archives of Razi Institute · 2023Article
- Effects of Dietary Supplementation of arginine-silicate- Inositol and Phytase Complex on Egg Quality, Egg Shell Strength, and Blood Biochemical Characteristics of Laying Hens.Archives of Razi Institute · 2023Article
- Experimental Testing of the Action of Vitamin D and Silicon Chelates in Bone Fracture Healing and Bone Turnover in Mice and Rats.Nutrients · 2022Article
- Dietary L-citrulline supplementation modulates nitric oxide synthesis and anti-oxidant status of laying hens during summer season.Journal of animal science and biotechnology · 2020Article
- Melatonin Improves Laying Performance by Enhancing Intestinal Amino Acids Transport in Hens.Frontiers in endocrinology · 2018Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effects of supplementation of arginine-silicate-inositol complex (ASI; 49.5-8.2-25 g/kg, respectively) to laying hens were investigated with respect to eggshell quality, calcium (Ca) balance, and expression of duodenal proteins related to Ca metabolism (calbindin and tight junction proteins). A total of 360 laying hens, 25 weeks old, were divided into 3 groups consisting of 6 replicate of cages, 20 birds per cage. The groups were fed a basal diet and the basal diet supplemented with 500 or 1000 mg ASI complex per kilogram for 90 days. Data were analyzed by ANCOVA using data during the first week of the adaptation period as covariates. As the ASI complex supplementation level increased, there were increases in feed intake (P < 0.0001), egg production (P < 0.001), egg weight (P < 0.0001) and eggshell weight (P < 0.001) weight, and shell thickness (P < 0.001) and decreases in feed conversion ratio and cracked egg percentage (P < 0.0001 for both). Concentrations of serum osteocalcin (P < 0.0001), vitamin D (P < 0.0001), calcium (P < 0.001), phosphorus (P < 0.001), and alkaline phosphatase (P < 0.008) as well as amounts of calcium retention (P < 0.0001) and eggshell calcium deposition (P < 0.001), and Ca balance (P < 0.0001) increased, whereas amount of calcium excretion (P < 0.001) decreased linearly in a dose-dependent manner. The ASI complex supplementation increased expressions of calcium transporters (calbindin-D28k, N sodium-calcium exchanger, plasma membrane calcium ATPase, and vitamin D receptor) and tight junction proteins (zonula occludens-1 and occludin) in the duodenum in a linear fashion (P < 0.0001 for all). In conclusion, provision of dietary ASI complex to laying hens during the peak laying period improved eggshell quality through improving calcium utilization as reflected by upregulation of genes related to the calcium metabolism. Further studies are needed to elucidate the contribution of each of the ASI complex ingredients.
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