ArticleOrganogenesis2018
Prolongation of liver-specific function for primary hepatocytes maintenance in 3D printed architectures.
Article in Organogenesis, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 39 citations in OpenAlex.
- Developing adverse outcome pathways underlying CAR activation-induced liver injuries using HepG2 spheroid model.Archives of toxicology · 2026Article
- Advances in Cryopreservation Strategies for 3D Biofabricated Constructs: From Hydrogels to Bioprinted Tissues.International journal of molecular sciences · 2025Review
- Liver organoids: Current advances and future applications for hepatology.Clinical and molecular hepatology · 2025Review
- 3D bioprinting of liver models: A systematic scoping review of methods, bioinks, and reporting quality.Materials today. Bio · 2024Article
- Application of 3D Bioprinting in Liver Diseases.Micromachines · 2023Review
- Advances of Engineered Hydrogel Organoids within the Stem Cell Field: A Systematic Review.Gels (Basel, Switzerland) · 2022Review
- Review
- A 3D primary human cell-based in vitro model of non-alcoholic steatohepatitis for efficacy testing of clinical drug candidates.Scientific reports · 2021Article
- Emerging liver organoid platforms and technologies.Cell regeneration (London, England) · 2021Review
- Human placental hydrolysate promotes the long-term culture of hepatocyte-like cells derived from canine bone marrow.The Journal of veterinary medical science · 2021Article
- 3D Bioprinting at the Frontier of Regenerative Medicine, Pharmaceutical, and Food Industries.Frontiers in medical technology · 2020Review
- Bioprinting for Liver Transplantation.Bioengineering (Basel, Switzerland) · 2019Review
- Development and validation of an in vitro 3D model of NASH with severe fibrotic phenotype.American journal of translational research · 2019Article
- Bioprinting with human stem cell-laden alginate-gelatin bioink and bioactive glass for tissue engineering.International journal of bioprinting · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isolated primary hepatocytes from the liver are very similar to in vivo native liver hepatocytes, but they have the disadvantage of a limited lifespan in 2D culture. Although a sandwich culture and 3D organoids with mesenchymal stem cells (MSCs) as an attractive assistant cell source to extend lifespan can be used, it cannot fully reproduce the in vivo architecture. Moreover, long-term 3D culture leads to cell death because of hypoxic stress. Therefore, to overcome the drawback of 2D and 3D organoids, we try to use a 3D printing technique using alginate hydrogels with primary hepatocytes and MSCs. The viability of isolated hepatocytes was more than 90%, and the cells remained alive for 7 days without morphological changes in the 3D hepatic architecture with MSCs. Compared to a 2D system, the expression level of functional hepatic genes and proteins was higher for up to 7 days in the 3D hepatic architecture. These results suggest that both the 3D bio-printing technique and paracrine molecules secreted by MSCs supported long-term culture of hepatocytes without morphological changes. Thus, this technique allows for widespread expansion of cells while forming multicellular aggregates, may be applied to drug screening and could be an efficient method for developing an artificial liver.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.