ArticleBiomedical optics express2018
Two-photon deep-tissue spatially resolved mitochondrial imaging using membrane potential fluorescence fluctuations.
Article in Biomedical optics express, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed.
- The Matrix of Mitochondrial Imaging: Exploring Spatial Dimensions.Biomolecules · 2025Review
- Compromised Muscle Properties in a Severe Hypophosphatasia Murine Model.International journal of molecular sciences · 2023Article
- Review
- Imaging mitochondria through bone in live mice using two-photon fluorescence microscopy with adaptive optics.Frontiers in neuroimaging · 2023Article
- In situ measurement of the isoplanatic patch for imaging through intact bone.Journal of biophotonics · 2021Article
- Spatial frequency metrics for analysis of microscopic images of musculoskeletal tissues.Connective tissue research · 2021Article
- Five-dimensional two-photon volumetric microscopy of in-vivo dynamic activities using liquid lens remote focusing.Biomedical optics express · 2019Article
- Nonlinear Imaging Histopathology: A Pipeline to Correlate Gold-Standard Hematoxylin and Eosin Staining With Modern Nonlinear Microscopy.IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics SocietyArticle
Corrections and comments
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Authors and funding
5 authors.
Funding
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Abstract
Cell metabolism and viability are directly reflected in their mitochondria. Imaging-based analysis of mitochondrial morphological structure, size and dynamic characteristics can therefore provide critical insight into cell function. However, mitochondria are often very abundant, and due to their close to diffraction-limit size, it is often non-trivial to distinguish a tubular or large mitochondrion from an ensemble of punctate mitochondria. In this paper, we use membrane potential dependent fluorescence fluctuations of individual mitochondria to resolve them using an approach similar to single molecule localization microscopy. We use 2-photon microscopy to image mitochondrial intensity fluctuations at 200 μm deep inside an intact in-vivo mouse soleus muscle. By analyzing the acquired images, we can reconstruct images with an extra layer of information about individual mitochondria, separated from their ensemble. Our analysis shows a factor of 14 improvement in detection of mitochondria.
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Registered trials
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