ArticleScientific reports2018
Deactivation of Cellulase at the Air-Liquid Interface Is the Main Cause of Incomplete Cellulose Conversion at Low Enzyme Loadings.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 82 citations in OpenAlex.
- Inactivation of PETase at Interfaces Inhibits PET Plastic Depolymerization.ACS sustainable chemistry & engineering · 2026Article
- Inactivation and process intensification of β-glucosidase in biomass utilization.Applied microbiology and biotechnology · 2023Review
- Surfactants, Biosurfactants, and Non-Catalytic Proteins as Key Molecules to Enhance Enzymatic Hydrolysis of Lignocellulosic Biomass.Molecules (Basel, Switzerland) · 2022Review
- Parametric optimization of the production of cellulose nanocrystals (CNCs) from South African corncobs via an empirical modelling approach.Scientific reports · 2022Article
- Living materials with programmable functionalities grown from engineered microbial co-cultures.Nature materials · 2021Article
- Microbubble enhanced mass transfer efficiency of CORSC advances · 2021Article
- Using poly(N-Vinylcaprolactam) to Improve the Enzymatic Hydrolysis Efficiency of Phenylsulfonic Acid-Pretreated Bamboo.Frontiers in bioengineering and biotechnology · 2021Article
- Effect of a Nonionic Surfactant on Enzymatic Hydrolysis of Lignocellulose Based on Lignocellulosic Features and Enzyme Adsorption.ACS omega · 2020Article
- Constraints and advances in high-solids enzymatic hydrolysis of lignocellulosic biomass: a critical review.Biotechnology for biofuels · 2020Review
- Considerations when Measuring Biocatalyst Performance.Molecules (Basel, Switzerland) · 2019Review
- Eco-friendly consolidated process for co-production of xylooligosaccharides and fermentable sugars using self-providing xylonic acid as key pretreatment catalyst.Biotechnology for biofuels · 2019Article
- Impacts of cellulase deactivation at the moving air-liquid interface on cellulose conversions at low enzyme loadings.Biotechnology for biofuels · 2019Article
- Promoting enzymatic hydrolysis of lignocellulosic biomass by inexpensive soy protein.Biotechnology for biofuels · 2019Article
- Ultrastructure and Enzymatic Hydrolysis of Deuterated Switchgrass.Scientific reports · 2018Article
- Real-time adsorption and action of expansin on cellulose.Biotechnology for biofuels · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Amphiphilic additives such as bovine serum albumin (BSA) and Tween have been used to improve cellulose hydrolysis by cellulases. However, there has been a lack of clarity to explain their mechanism of action in enzymatic hydrolysis of pure or low-lignin cellulosic substrates. In this work, a commercial Trichoderma reesei enzyme preparation and the amphiphilic additives BSA and Tween 20 were applied for hydrolysis of pure Avicel cellulose. The results showed that these additives only had large effects on cellulose conversion at low enzyme to substrate ratios when the reaction flasks were shaken. Furthermore, changes in the air-liquid interfacial area profoundly affected cellulose conversion, but surfactants reduced or prevented cellulase deactivation at the air-liquid interface. Not shaking the flasks or adding low amounts of surfactant resulted in near theoretical cellulose conversion at low enzyme loadings given enough reaction time. At low enzyme loadings, hydrolysis of cellulose in lignocellulosic biomass with low lignin content suffered from enhanced enzyme deactivation at the air-liquid interface.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.