ArticleNature cell biology2018
Affinity purification of cell-specific mitochondria from whole animals resolves patterns of genetic mosaicism.
Article in Nature cell biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Lens tissue transcriptome analysis in patients with total congenital cataract.Human genomics · 2026Article
- The microRNA miR-71 suppresses maladaptive UPRNature communications · 2025Article
- Leucine inhibits degradation of outer mitochondrial membrane proteins to adapt mitochondrial respiration.Nature cell biology · 2025Article
- Mitochondrial stress orchestrates chromatin remodeling and longevity via phosphoregulation of the NuRD component LIN-40.Science China. Life sciences · 2025Article
- Mitochondrial curation for the next generation.Current opinion in genetics & development · 2025Review
- Mitochondrial Metabolomics in Cancer: Mass Spectrometry-Based Approaches for Metabolic Rewiring Analysis and Therapeutic Discovery.Metabolites · 2025Review
- Article
- Novel imaging tools to study mitochondrial morphology inLife science alliance · 2024Article
- Protein kinase 2 of the giant sarcomeric protein UNC-89 regulates mitochondrial morphology and function.Communications biology · 2024Article
- Endogenous hydrogen peroxide positively regulates secretion of a gut-derived peptide in neuroendocrine potentiation of the oxidative stress response inbioRxiv : the preprint server for biology · 2024Article
- Multiple distinct evolutionary mechanisms govern the dynamics of selfish mitochondrial genomes in Caenorhabditis elegans.Nature communications · 2024Article
- The germline coordinates mitokine signaling.Cell · 2024Article
- Olfaction regulates peripheral mitophagy and mitochondrial function.Science advances · 2024Article
- [Preparation of peptide-functionalized affinity materials for the highly specific capture and analysis of mitochondria].Se pu = Chinese journal of chromatography · 2024Article
- Friend turned foe: selfish behavior of a spontaneously arising mitochondrial deletion in an experimentally evolved Caenorhabditis elegans population.G3 (Bethesda, Md.) · 2024Article
- Mitochondrial GTP metabolism controls reproductive aging in C. elegans.Developmental cell · 2023Article
- Review
- ATFS-1 counteracts mitochondrial DNA damage by promoting repair over transcription.Nature cell biology · 2023Article
- Evolutionary codependency: insights into the mitonuclear interaction landscape from experimental and wild Caenorhabditis nematodes.Current opinion in genetics & development · 2023Review
- Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although mitochondria are ubiquitous organelles, they exhibit tissue-specific morphology, dynamics and function. Here, we describe a robust approach to isolate mitochondria from specific cells of diverse tissue systems in Caenorhabditis elegans. Cell-specific mitochondrial affinity purification (CS-MAP) yields intact and functional mitochondria with exceptional purity and sensitivity (>96% enrichment, >96% purity, and single-cell and single-animal resolution), enabling comparative analyses of protein and nucleic acid composition between organelles isolated from distinct cellular lineages. In animals harbouring a mixture of mutant and wild-type mitochondrial genomes, we use CS-MAP to reveal subtle mosaic patterns of cell-type-specific heteroplasmy across large populations of animals (>10,000 individuals). We demonstrate that the germline is more prone to propagating deleterious mitochondrial genomes than somatic lineages, which we propose is caused by enhanced mtDNA replication in this tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.