ArticleProceedings of the National Academy of Sciences of the United States of America2018
Aberration-corrected cryoimmersion light microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 43 citations in OpenAlex.
- Challenges and limitations for live cell imaging in extreme cold.Methods and applications in fluorescence · 2026Article
- Time-deterministic cryo-optical microscopy.Light, science & applications · 2025Article
- Cryogenic single-molecule fluorescence imaging.BMB reports · 2025Review
- Cryo-electron tomography on focused ion beam lamellae transforms structural cell biology.Nature methods · 2023Review
- IL-10-providing B cells govern pro-inflammatory activity of macrophages and microglia in CNS autoimmunity.Acta neuropathologica · 2023Article
- Ultrarapid cryo-arrest of living cells on a microscope enables multiscale imaging of out-of-equilibrium molecular patterns.Science advances · 2021Article
- CorRelator: Interactive software for real-time high precision cryo-correlative light and electron microscopy.Journal of structural biology · 2021Article
- Challenges facing quantitative large-scale optical super-resolution, and some simple solutions.iScience · 2021Review
- Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodies.Communications biology · 2021Article
- Liquid electron microscopy: then, now and future.Applied microscopy · 2019Review
- Hybrid sequencing-based personal full-length transcriptomic analysis implicates proteostatic stress in metastatic ovarian cancer.Oncogene · 2019Article
- Cryo-SOFI enabling low-dose super-resolution correlative light and electron cryo-microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Correlative cryo super-resolution light and electron microscopy on mammalian cells using fluorescent proteins.Scientific reports · 2019Article
- Solid immersion microscopy images cells under cryogenic conditions with 12 nm resolution.Communications biology · 2019Article
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Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryogenic fluorescent light microscopy of flash-frozen cells stands out by artifact-free fixation and very little photobleaching of the fluorophores used. To attain the highest level of resolution, aberration-free immersion objectives with accurately matched immersion media are required, but both do not exist for imaging below the glass-transition temperature of water. Here, we resolve this challenge by combining a cryoimmersion medium, HFE-7200, which matches the refractive index of room-temperature water, with a technological concept in which the body of the objective and the front lens are not in thermal equilibrium. We implemented this concept by replacing the metallic front-lens mount of a standard bioimaging water immersion objective with an insulating ceramic mount heated around its perimeter. In this way, the objective metal housing can be maintained at room temperature, while creating a thermally shielded cold microenvironment around the sample and front lens. To demonstrate the range of potential applications, we show that our method can provide superior contrast in
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.