Evidence map›Paper›PMID 29355496›Full record

ArticleExperimental parasitology2018

The glycosomal alkyl-dihydroxyacetonephosphate synthase TbADS is essential for the synthesis of ether glycerophospholipids in procyclic trypanosomes.

Sungsu Lee, Melanie Cheung-See-Kit, Tyler A Williams, Nader Yamout, Rachel Zufferey

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental parasitology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Sungsu LeeDepartment of Biological Sciences, St John's University, 8000 Utopia Parkway, Jamaica, NY 11439, USA.
Melanie Cheung-See-KitDepartment of Biological Sciences, St John's University, 8000 Utopia Parkway, Jamaica, NY 11439, USA.
Tyler A WilliamsDepartment of Biological Sciences, St John's University, 8000 Utopia Parkway, Jamaica, NY 11439, USA.
Nader YamoutDepartment of Biological Sciences, St John's University, 8000 Utopia Parkway, Jamaica, NY 11439, USA.
Rachel ZuffereyDepartment of Biological Sciences, St John's University, 8000 Utopia Parkway, Jamaica, NY 11439, USA. Electronic address: zufferer@stjohns.edu.
St. John's University · US

Funding

UNDERSTANDING THE ROLE OF SMALL RNAS IN ARABIDOPSIS POLLEN DEVELOPMENTP20RR016475 · NCRR · UNIVERSITY OF KANSAS MEDICAL CENTER · PI LUSHINGTON, GERALD H · 2001 to 2011
$38.1M
"TRAINing' For Success in Biomedical Research CareersT36GM101995 · NIGMS · BROWN UNIVERSITY · PI CAMPBELL, ANDREW G. · 2013 to 2015
$725k
Fatty Alcohol Synthesis and Virulence in LeishmaniaSC3GM113743 · NIGMS · ST. JOHN'S UNIVERSITY · PI ZUFFEREY, RACHEL · 2015 to 2018
$495k
NCRR NIH HHS P20 RR016475NIGMS NIH HHS SC3 GM113743NIGMS NIH HHS T36 GM101995
6 · The paper itself

Abstract

Glycerophospholipids are the main constituents of the biological membranes in Trypanosoma brucei, which causes sleeping sickness in humans. The present work reports the characterization of the alkyl-dihydroxyacetonephosphate synthase TbADS that catalyzes the committed step in ether glycerophospholipid biosynthesis. TbADS localizes to the glycosomal lumen. TbADS complemented a null mutant of Leishmania major lacking alkyl-dihydroxyacetonephosphate synthase activity and restored the formation of normal form of the ether lipid based virulence factor lipophosphoglycan. Despite lacking alkyl-dihydroxyacetonephosphate synthase activity, a null mutant of TbADS in procyclic trypanosomes remained viable and exhibited normal growth. Comprehensive analysis of cellular glycerophospholipids showed that TbADS was involved in the biosynthesis of all ether glycerophospholipid species, primarily found in the PE and PC classes.

Indexed as

Alkyl and Aryl TransferasesGlycerophospholipidsLeishmania majorLoss of Function MutationMicrobodiesPlasmidsTandem Mass SpectrometryTrypanosoma brucei bruceiAlkyl and Aryl Transferasesalkylglycerone-phosphate synthaseGlycerophospholipidsAlkyl-dihydroxyacetonephosphate synthaseEther glycerophospholipidsGlycosomeNull mutantTrypanosoma brucei

Identifiers

PMID29355496
PMCPMC5801073
OpenAlexW2790508294

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.