Evidence map›Paper›PMID 29355143›Full record

ArticleThe Indian journal of medical research2017

Value of CD16/CD66b/CD45 in comparison to CD55/CD59/CD45 in diagnosis of paroxysmal nocturnal haemoglobinuria: An Indian experience.

Mrinalini Kotru, Rahul Sharma, Suman Kumar Pramanik, Abhishek Purohit, Gurmeet Singh, Avinash Kumar Singh, Deepti Muterja, Pravas Mishra, Tulika Seth, Seema Tyagi and 3 more

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In one paragraph

Article in The Indian journal of medical research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 81% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 1 institution in 1 country.

Mrinalini KotruDepartment of Hematology, All India Institute of Medical Sciences, New Delhi, India.
Rahul SharmaDepartment of Hematology, All India Institute of Medical Sciences, New Delhi, India.
Suman Kumar PramanikDepartment of Hematology, All India Institute of Medical Sciences, New Delhi, India.
Abhishek PurohitDepartment of Hematology, All India Institute of Medical Sciences, New Delhi, India.
Gurmeet SinghDepartment of Hematology, All India Institute of Medical Sciences, New Delhi, India.
Avinash Kumar SinghDepartment of Hematology, All India Institute of Medical Sciences, New Delhi, India.
Deepti MuterjaDepartment of Hematology, All India Institute of Medical Sciences, New Delhi, India.
Pravas MishraDepartment of Hematology, All India Institute of Medical Sciences, New Delhi, India.
Tulika SethDepartment of Hematology, All India Institute of Medical Sciences, New Delhi, India.
Seema TyagiDepartment of Hematology, All India Institute of Medical Sciences, New Delhi, India.
Manoranjan MahapatraDepartment of Hematology, All India Institute of Medical Sciences, New Delhi, India.
Hara Prasad PatiDepartment of Hematology, All India Institute of Medical Sciences, New Delhi, India.
Renu SaxenaDepartment of Hematology, All India Institute of Medical Sciences, New Delhi, India.
All India Institute of Medical Sciences · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND &

objectivesDiagnosis of paroxysmal nocturnal haemoglobinuria (PNH), a rare haematopoietic stem cell disorder, is challenging in patients with bone marrow failure (BMF) syndrome like aplastic anaemia (AA). This study was conducted with the aim to test the efficacy of the newly recommended markers viz. anti-CD16 and CD66b antibody over the existing anti-CD55 and CD59 antibody for PNH diagnosis in India.

methodsThis study was conducted on 193 suspected cases of PNH by flow cytometry using lyse wash technique to stain the granulocytes with CD16/CD66b and CD55/CD59.

resultsOf the 193 suspected cases, 62 patients showed the presence of PNH clone. Forty six patients were detected by CD55/CD59/CD45, whereas 61 were detected by CD16/CD66b/CD45. CD16/CD66b detected 16 (25.8%) additional patients over CD55/CD59 (P<0.05) and was more sensitive in detecting the PNH clone with higher negative predictive value. Most of the patients (11/16) who were picked up by CD16/CD66b were of AA who had small clone sizes. Further, the PNH clones were more discreetly identified in CD16/CD66b plots than by CD55/CD59. Clone size assessed by CD16/CD66b which reflects the clinical severity of classical PNH (thrombosis/haemolysis), was more representative of the underlying clinical condition than CD55/59. INTERPRETATION &

conclusionsIn our experience of 62 patients of PNH, CD16/CD66b proved to be more efficacious in detecting PNH. The new panel was especially useful in monitoring PNH associated with BMF which had small clone sizes.

Indexed as

AdultAnemia, AplasticAntibodies, Anti-IdiotypicAntigens, CDBone Marrow DiseasesBone Marrow Failure DisordersCD55 AntigensCD59 AntigensCell Adhesion MoleculesFemaleFlow CytometryGPI-Linked ProteinsHemoglobinuria, ParoxysmalHumansLeukocyte Common AntigensMaleAntibodies, Anti-IdiotypicAntigens, CDCD55 AntigensCD59 AntigensCEACAM8 protein, humanCell Adhesion MoleculesFCGR3B protein, humanGPI-Linked ProteinsLeukocyte Common AntigensReceptors, IgG

Identifiers

PMID29355143
PMCPMC5793471
OpenAlexW2792705215

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.