ArticleTissue engineering. Part C, Methods2018
Live Quantitative Monitoring of Mineral Deposition in Stem Cells Using Tetracycline Hydrochloride.
Article in Tissue engineering. Part C, Methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 32 citations in OpenAlex.
- Distinct Osteogenic Profiles of Tetracyclines from Different Generations in an Ex Vivo Embryonic Chick Femur Model.International journal of molecular sciences · 2026Article
- Developing a Bone-Mimicking Microenvironment: Surface Coating Method for Investigating Bone RemodelingACS biomaterials science & engineering · 2025Article
- LL-37 regulates odontogenic differentiation of dental pulp stem cells in an inflammatory microenvironment.Stem cell research & therapy · 2024Article
- Effects of Daidzein, Tempeh, and a Probiotic Digested in an Artificial Gastrointestinal Tract on Calcium Deposition in Human Osteoblast-like Saos-2 Cells.International journal of molecular sciences · 2024Article
- Unveiling the Osteogenic Potential of Tetracyclines: A Comparative Study in Human Mesenchymal Stem Cells.Cells · 2023Article
- Host defense peptides combined with MTA extract increase the repair in dental pulp cells: in vitro and ex vivo study.Scientific reports · 2023Article
- Simultaneous Labeling of Adipogenic and Osteogenic Differentiating Stem Cells for Live Confocal Analysis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A critical review of in vitro research methodologies used to study mineralization in human dental pulp cell cultures.International endodontic journal · 2022Review
- Models and Techniques to Study Aortic Valve CalcificationFrontiers in pharmacology · 2022Review
- Article
- Stage-Dependent Regulation of Dental Pulp Stem Cell Odontogenic Differentiation by Transforming Growth Factor-Stem cells international · 2022Article
- Fisetin Inhibits Osteogenic Differentiation of Mesenchymal Stem Cells via the Inhibition of YAP.Antioxidants (Basel, Switzerland) · 2021Article
- A specific aggregation-induced emission-conjugated polymer enables visual monitoring of osteogenic differentiation.Bioactive materials · 2020Article
- Article
- Aspiration-assisted bioprinting of the osteochondral interface.Scientific reports · 2020Article
- Role of nanoparticles in osteogenic differentiation of bone marrow mesenchymal stem cells.Cytotechnology · 2020Article
- Spectroscopic Methods Used in Implant Material Studies.Molecules (Basel, Switzerland) · 2020Review
- Inhibited Maternal Bone Resorption Suppress Fetal Rat Bone Development During Pregnancy.Frontiers in cell and developmental biology · 2020Article
- Live Simultaneous Monitoring of Mineral Deposition and Lipid Accumulation in Differentiating Stem Cells.Biomimetics (Basel, Switzerland) · 2019Article
- Magnetically Assisted Control of Stem Cells Applied in 2D, 3D and In Situ Models of Cell Migration.Molecules (Basel, Switzerland) · 2019Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The final stage of in vitro osteogenic differentiation is characterized by the production of mineral deposits containing calcium cations and inorganic phosphates, which populate the extracellular matrix (ECM) surrounding the cell monolayer. Conventional histological techniques for the assessment of mineralization, such as Von Kossa and Alizarin Red S staining, are end point techniques requiring cell fixation. Moreover, in both cases staining quantitation requires dye extraction, which irreversibly alters the ECM conformation and structure, therefore preventing the use of the sample for further analysis. In this study, the use of tetracycline hydrochloride (TC) is proposed for the nondestructive staining, quantitation, and imaging of mineralizing bone-like nodules in live cultures of human bone marrow mesenchymal stem cells cultured under osteogenic conditions. Overnight administration of TC to living cells was shown not to alter the metabolic activity or the progression of cell differentiation. When applied to differentiating cultures, cell exposure to serial doses of TC was found to produce quantifiable fluorescence emission specifically in osteogenic cultures. Incubation with TC enabled fluorescence imaging of mineralized areas in live cultures and the combination with other fluorophores using appropriate filters. These results demonstrate that serial TC administration over the differentiation time course provides a qualitative and quantitative tool for the monitoring and evaluation of the differentiation process in live cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.