ArticleDevelopment genes and evolution2018
Transgenesis by microparticle bombardment for live imaging of fluorescent proteins in Pristionchus pacificus germline and early embryos.
Article in Development genes and evolution, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 16 citations in OpenAlex.
- Programmed DNA elimination was present in the last common ancestor ofbioRxiv : the preprint server for biology · 2026Article
- Characterization of Heat Shock Protein Expression and Its Application to Temporal Gene Expression in the Nematode Pristionchus pacificus.Development, growth & differentiation · 2026Article
- The rewiring of a terminal selector regulatory cascade generates convergent neuronal laterality.PLoS genetics · 2026Article
- Microparticle Bombardment as a Method for Transgenesis inmicroPublication biology · 2025Article
- Visualization of gene expression inmicroPublication biology · 2024Article
- Genomic integration of transgenes using UV irradiation inmicroPublication biology · 2022Article
- Transgenesis of the gonochoristic nematodemicroPublication biology · 2022Article
- Analysis of meiosis ineLife · 2021Article
- Different combinations of serotonin receptors regulate predatory and bacterial feeding behaviors in the nematode Pristionchus pacificus.G3 (Bethesda, Md.) · 2021Article
- Crowdsourcing and the feasibility of manual gene annotation: A pilot study in the nematode Pristionchus pacificus.Scientific reports · 2019Article
- An antibody staining protocol variation for nematodes that adds heat-induced antigen retrieval (HIAR).microPublication biology · 2019Article
- From "the Worm" to "the Worms" and Back Again: The Evolutionary Developmental Biology of Nematodes.Genetics · 2018Review
- Streptothricin acetyl transferase 2 (Sat2): A dominant selection marker for Caenorhabditis elegans genome editing.PloS one · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Pristionchus pacificus is a free-living nematode used as a model organism for evolutionary developmental and ecological biology. Although a transgenic technique to form complex arrays by microinjection has been established in P. pacificus, transgene expression from the array in the germline and early embryos tends to be silenced. Here, we established a method to integrate transgenes into the genome of P. pacificus using microparticle bombardment with hygromycin B selection. Additionally, we isolated a mutant exhibiting significantly lower autofluorescence in the germline and early embryos, facilitating visualization of transgene-derived fluorescent proteins for live imaging. Transgenic lines constructed using these tools successfully expressed GFP-tagged proteins in the germline and early embryos and enabled live imaging of chromosomes, microtubules, and centrosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.