Evidence map›Paper›PMID 29353239›Full record

ArticleJournal of lipid research2018

Altered eicosanoid production and phospholipid remodeling during cell culture.

Toshiaki Okuno, Miguel A Gijón, Simona Zarini, Sarah A Martin, Robert M Barkley, Christopher A Johnson, Mai Ohba, Takehiko Yokomizo, Robert C Murphy

Open access · hybridAbstract read
In one paragraph

Article in Journal of lipid research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
2.3field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 19 citations in OpenAlex.

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  8. Prostaglandin EGenes to cells : devoted to molecular & cellular mechanisms · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Toshiaki OkunoDepartment of Biochemistry, Juntendo University School of Medicine, Bunkyo-ku, Tokyo 113-8421, Japan.ORCID 0000-0003-0270-5744
Miguel A GijónDepartment of Pharmacology, Anschutz Medical Campus, University of Colorado Denver, Aurora, CO 80045.
Simona ZariniDepartment of Pharmacology, Anschutz Medical Campus, University of Colorado Denver, Aurora, CO 80045.ORCID 0000-0002-7460-3299
Sarah A MartinDepartment of Pharmacology, Anschutz Medical Campus, University of Colorado Denver, Aurora, CO 80045.
Robert M BarkleyDepartment of Pharmacology, Anschutz Medical Campus, University of Colorado Denver, Aurora, CO 80045.
Christopher A JohnsonDepartment of Pharmacology, Anschutz Medical Campus, University of Colorado Denver, Aurora, CO 80045.
Mai OhbaDepartment of Biochemistry, Juntendo University School of Medicine, Bunkyo-ku, Tokyo 113-8421, Japan.
Takehiko YokomizoDepartment of Biochemistry, Juntendo University School of Medicine, Bunkyo-ku, Tokyo 113-8421, Japan.
Robert C MurphyDepartment of Pharmacology, Anschutz Medical Campus, University of Colorado Denver, Aurora, CO 80045 Robert.Murphy@ucdenver.edu.ORCID 0000-0002-9430-6515
University of Colorado Anschutz Medical Campus · USJuntendo University · JP

Funding

Pilot Study: POPULATION PHARMACOLOGYU54HL117798 · NHLBI · UNIVERSITY OF PENNSYLVANIA · PI BLAIR, IAN ALEXANDER · 2012 to 2016
$18.5M
Determine the Role of Lipid Mediators in Secondary Traumatic Brain InjuryF31NS080486 · NINDS · UNIVERSITY OF COLORADO DENVER · PI MARTIN, SARAH ASHLEY · 2013 to 2014
$58k
NHLBI NIH HHS U54 HL117798NINDS NIH HHS F31 NS080486
6 · The paper itself

Abstract

The remodeling of PUFAs by the Lands cycle is responsible for the diversity of phospholipid molecular species found in cells. There have not been detailed studies of the alteration of phospholipid molecular species as a result of serum starvation or depletion of PUFAs that typically occurs during tissue culture. The time-dependent effect of cell culture on phospholipid molecular species in RAW 264.7 cells cultured for 24, 48, or 72 h was examined by lipidomic strategies. These cells were then stimulated to produce arachidonate metabolites derived from the cyclooxygenase pathway, thromboxane B

Indexed as

Cell Culture TechniquesAnimalsChromatography, High Pressure LiquidEicosanoidsMicePhospholipidsRAW 264.7 CellsTandem Mass SpectrometryTumor Necrosis Factor-alphaEicosanoidsPhospholipidsTumor Necrosis Factor-alphaMead acidpolyunsaturated fatty acidvolcano plot

Identifiers

PMID29353239
PMCPMC5832926
OpenAlexW2784347781

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.