Evidence map›Paper›PMID 29352327›Full record

ArticleVirchows Archiv : an international journal of pathology2018

LIMK/cofilin pathway and Slingshot are implicated in human colorectal cancer progression and chemoresistance.

Helen Aggelou, Panagiota Chadla, Sofia Nikou, Sofia Karteri, Ioannis Maroulis, Haralabos P Kalofonos, Helen Papadaki, Vasiliki Bravou

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In one paragraph

Article in Virchows Archiv : an international journal of pathology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed, 1 pooled it
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Helen AggelouDepartment of Anatomy-Histology-Embryology, Medical School, University of Patras, 26500, Patras, Greece.
Panagiota ChadlaDepartment of Anatomy-Histology-Embryology, Medical School, University of Patras, 26500, Patras, Greece.
Sofia NikouDepartment of Anatomy-Histology-Embryology, Medical School, University of Patras, 26500, Patras, Greece.
Sofia KarteriDepartment of Anatomy-Histology-Embryology, Medical School, University of Patras, 26500, Patras, Greece.
Ioannis MaroulisDepartment of Surgery, University of Patras Medical School, Patras, Greece.
Haralabos P KalofonosDivision of Oncology, University of Patras Medical School, Patras, Greece.
Helen PapadakiDepartment of Anatomy-Histology-Embryology, Medical School, University of Patras, 26500, Patras, Greece.
Vasiliki BravouDepartment of Anatomy-Histology-Embryology, Medical School, University of Patras, 26500, Patras, Greece. vibra@upatras.gr.ORCID http://orcid.org/0000-0002-4635-0204
University of Patras · GR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cofilin phospho-regulation is important for actin filament turnover and is implicated in cancer. Phosphorylation of cofilin is mediated by LIM kinases (LIMKs) and dephosphorylation by Slingshot phosphatases (SSH). LIMKs and SSH promote cancer cell invasion and metastasis and represent novel anti-cancer targets. However, little is known regarding LIMK/cofilin and SSH in human colorectal cancer (CRC). In this study, we aimed to address their expression and significance in human CRC. We evaluated expression of non-phosphorylated (active) and phosphorylated cofilin, LIMK1, LIMK2, and SSH1 by immunohistochemistry in 143 human CRC samples in relation to clinicopathologic parameters, response of metastatic disease to chemotherapy, and epithelial-mesenchymal transition (EMT) markers β-catenin, E-cadherin, and ZEB. We show that active cofilin, LIMK1, LIMK2, and SSH1 are overexpressed in human CRC and are associated with tumor progression parameters. SSH1 is an independent predictor of lymph node metastasis by multivariate analysis. LIMK1 and SSH1 expression is also higher in non-responders to chemotherapy, and SSH1 is shown by multivariate analysis to independently predict response of metastatic disease to chemotherapy. Active cofilin, LIMK1, LIMK2, and SSH1 also correlated with the EMT markers examined. In addition, immunofluorescence analysis showed increased expression of active cofilin, LIMK1, LIMK2, and SSH1 in HT29 colon cancer cells resistant to 5-fluorouracil compared to parental HT29 cells. Our results suggest that F-actin regulators LIMK/cofilin pathway and SSH1 are associated with CRC progression and chemoresistance representing promising tumor biomarkers and therapeutic targets in CRC.

Indexed as

Actin Depolymerizing FactorsAdultAgedAged, 80 and overAntineoplastic Combined Chemotherapy ProtocolsBiomarkers, TumorColorectal NeoplasmsDisease ProgressionDrug Resistance, NeoplasmFemaleHumansLim KinasesMaleMiddle AgedPhosphoprotein PhosphatasesSignal TransductionActin Depolymerizing FactorsBiomarkers, TumorLIMK1 protein, humanLIMK2 protein, humanLim KinasesPhosphoprotein PhosphatasesSSH1 protein, humanChemoresistanceCofilinColorectal cancerEMTLIMKSlingshot

Identifiers

PMID29352327
OpenAlexW2784900696

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.