ArticleThe Journal of cell biology2018
Spatiotemporal organization of exocytosis emerges during neuronal shape change.
Article in The Journal of cell biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 60 citations in OpenAlex.
- ExoFILT: transfer learning for robust and accelerated analysis of exocytosis single-particle tracking data.Bioinformatics (Oxford, England) · 2026Article
- Deciphering the Nanoscale Architecture of Presynaptic Actin Using a Micropatterned Presynapse-on-Glass Model.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Deep learning detection of dynamic exocytosis events in fluorescence TIRF microscopy.PLoS computational biology · 2025Article
- Forebrain neural progenitors effectively integrate into host brain circuits and improve neural function after ischemic stroke.Nature communications · 2025Article
- TRIM9 Controls Growth Cone Responses to Netrin Through DCC and UNC5C.Journal of neurochemistry · 2025Article
- pHusion - a robust and versatile toolset for automated detection and analysis of exocytosis.Journal of cell science · 2024Article
- Increased vesicular dynamics and nanoscale clustering of IL-2 after T cell activation.Biophysical journal · 2024Article
- Trafficking in cancer: from gene deregulation to altered organelles and emerging biophysical properties.Frontiers in cell and developmental biology · 2024Review
- Photomanipulation of Minimal Synthetic Cells: Area Increase, Softening, and Interleaflet Coupling of Membrane Models Doped with Azobenzene-Lipid Photoswitches.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Exosome secretion kinetics are controlled by temperature.Biophysical journal · 2023Article
- Spatial organization of lysosomal exocytosis relies on membrane tension gradients.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The premetazoan ancestry of the synaptic toolkit and appearance of first neurons.Essays in biochemistry · 2022Article
- CNS myelination requires VAMP2/3-mediated membrane expansion in oligodendrocytes.Nature communications · 2022Article
- Microglial control of neuronal development via somatic purinergic junctions.Cell reports · 2022Article
- Automated Detection and Analysis of Exocytosis.Journal of visualized experiments : JoVE · 2021Article
- Article
- The TRIM9/TRIM67 neuronal interactome reveals novel activators of morphogenesis.Molecular biology of the cell · 2021Article
- TRIM67 regulates exocytic mode and neuronal morphogenesis via SNAP47.Cell reports · 2021Article
- The ubiquitylome of developing cortical neurons.microPublication biology · 2020Article
- Tracking Calcium Dynamics and Immune Surveillance at the Choroid Plexus Blood-Cerebrospinal Fluid Interface.Neuron · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Neurite elongation and branching in developing neurons requires plasmalemma expansion, hypothesized to occur primarily via exocytosis. We posited that exocytosis in developing neurons and nonneuronal cells would exhibit distinct spatiotemporal organization. We exploited total internal reflection fluorescence microscopy to image vesicle-associated membrane protein (VAMP)-pHluorin-mediated exocytosis in mouse embryonic cortical neurons and interphase melanoma cells, and developed computer-vision software and statistical tools to uncover spatiotemporal aspects of exocytosis. Vesicle fusion behavior differed between vesicle types, cell types, developmental stages, and extracellular environments. Experiment-based mathematical calculations indicated that VAMP2-mediated vesicle fusion supplied excess material for the plasma membrane expansion that occurred early in neuronal morphogenesis, which was balanced by clathrin-mediated endocytosis. Spatial statistics uncovered distinct spatiotemporal regulation of exocytosis in the soma and neurites of developing neurons that was modulated by developmental stage, exposure to the guidance cue netrin-1, and the brain-enriched ubiquitin ligase tripartite motif 9. In melanoma cells, exocytosis occurred less frequently, with distinct spatial clustering patterns.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.