ArticleProceedings of the National Academy of Sciences of the United States of America2018
Full molecular trajectories of RNA polymerase at single base-pair resolution.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- The ribosome derives the energy to translocate and unwind mRNA from EF-G binding.Nature communications · 2025Article
- Autoregressive HMM resolves biomolecular transitions from passive optical tweezer force measurements.Biophysical journal · 2025Article
- Article
- Measuring molecular forces inside living cells using magnetic tweezers.Biophysical reviews · 2025Review
- Pleomorphic effects of three small-molecule inhibitors on transcription elongation bybioRxiv : the preprint server for biology · 2025Article
- Single-molecule dynamic structural biology with vertically arranged DNA on a fluorescence microscope.Nature methods · 2025Article
- Nanopore tweezers show fractional-nucleotide translocation in sequence-dependent pausing by RNA polymerase.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- DNA Origami: Recent Progress and Applications.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Step-by-Step Regulation of Productive and Abortive Transcription Initiation by Pyrophosphorolysis.Journal of molecular biology · 2022Article
- Optical manipulation: advances for biophotonics in the 21st century.Journal of biomedical optics · 2021Review
- A DNA packaging motor inchworms along one strand allowing it to adapt to alternative double-helical structures.Nature communications · 2021Article
- Residence time analysis of RNA polymerase transcription dynamics: A Bayesian sticky HMM approach.Biophysical journal · 2021Article
- DNA replication:Computational and structural biotechnology journal · 2021Review
- Optical tweezers in single-molecule biophysics.Nature reviews. Methods primers · 2021Article
- Observation of processive telomerase catalysis using high-resolution optical tweezers.Nature chemical biology · 2020Article
- The Mechanisms of Substrate Selection, Catalysis, and Translocation by the Elongating RNA Polymerase.Journal of molecular biology · 2019Review
- High-resolution and high-accuracy topographic and transcriptional maps of the nucleosome barrier.eLife · 2019Article
- The Role of Pyrophosphorolysis in the Initiation-to-Elongation Transition by E. coli RNA Polymerase.Journal of molecular biology · 2019Article
- Super-resolution force spectroscopy reveals ribosomal motion at sub-nucleotide steps.Chemical communications (Cambridge, England) · 2018Article
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Authors and funding
8 authors.
Funding
Abstract
In recent years, highly stable optical tweezers systems have enabled the characterization of the dynamics of molecular motors at very high resolution. However, the motion of many motors with angstrom-scale dynamics cannot be consistently resolved due to poor signal-to-noise ratio. Using an acousto-optic deflector to generate a "time-shared" dual-optical trap, we decreased low-frequency noise by more than one order of magnitude compared with conventional dual-trap optical tweezers. Using this instrument, we implemented a protocol that synthesizes single base-pair trajectories, which are used to test a Large State Space Hidden Markov Model algorithm to recover their individual steps. We then used this algorithm on real transcription data obtained in the same instrument to fully uncover the molecular trajectories of
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.