ArticleBMC microbiology2018
Phosphate signaling through alternate conformations of the PstSCAB phosphate transporter.
Article in BMC microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 86 citations in OpenAlex.
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- Bacterial metabolic remodeling by convergent evolution unlocks nutrient availability after a host switch.Science advances · 2026Article
- Molecular mechanism of phosphate import by the bacterial PstSCAB transporter.Nature communications · 2026Article
- Roles of Pho regulon in bacterial pathogenicity.Virulence · 2025Review
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- The adjacent ATP-binding protein-encoding genes of theJournal of bacteriology · 2025Article
- Revealing the Existence of Diverse Strategies for Phosphorus Solubilization and Acquisition in Plant-Growth PromotingMicroorganisms · 2025Article
- Universal receptive system as a novel regulator of transcriptomic activity of Staphylococcus aureus.Microbial cell factories · 2025Article
- Differential gene expression analysis shows that cephalosporin resistance is intrinsic to Clostridioides difficile strain 630.The Journal of antibiotics · 2025Article
- Small DUF1127 proteins regulate bacterial phosphate metabolism through protein-protein interactions with the sensor kinase PhoR.microLife · 2025Article
- First whole genome sequencing ofOpen veterinary journal · 2024Article
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- An intracellular phosphorus-starvation signal activates the PhoB/PhoR two-component system inmBio · 2024Article
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- The cytoplasmic phosphate level has a central regulatory role in the phosphate starvation response of Caulobacter crescentus.Communications biology · 2024Article
- Transcriptome Analysis of Protocatechualdehyde againstFoods (Basel, Switzerland) · 2023Article
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Authors and funding
5 authors at 1 institution in 1 country.
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Abstract
backgroundPhosphate is an essential compound for life. Escherichia coli employs a signal transduction pathway that controls the expression of genes that are required for the high-affinity acquisition of phosphate and the utilization of alternate sources of phosphorous. These genes are only expressed when environmental phosphate is limiting. The seven genes for this signaling pathway encode the two-component regulatory proteins PhoB and PhoR, as well as the high-affinity phosphate transporter PstSCAB and an auxiliary protein called PhoU. As the sensor kinase PhoR has no periplasmic sensory domain, the mechanism by which these cells sense environmental phosphate is not known. This paper explores the hypothesis that it is the alternating conformations of the PstSCAB transporter which are formed as part of the normal phosphate transport cycle that signal phosphate sufficiency or phosphate limitation.
resultsWe tested two variants of PstB that are predicted to lock the protein in either of two conformations for their signaling output. We observed that the pstBQ160K mutant, predicted to reside in an inward-facing, open conformation signaled phosphate sufficiency whereas the pstBE179Q mutant, predicted to reside in an outward-facing, closed conformation signaled phosphate starvation. Neither mutant showed phosphate transport.
conclusionsThese results support the hypothesis that the alternating conformations of the PstSCAB transporter are sensed by PhoR and PhoU. This sensory mechanism thus controls the alternate autokinase and phospho-PhoB phosphatase activities of PhoR, which ultimately control the signaling state of the response regulator PhoB.
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