ArticlePloS one2018
Omega-3 fatty acid desaturase gene family from two ω-3 sources, Salvia hispanica and Perilla frutescens: Cloning, characterization and expression.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed.
- Selection and Validation of Reference Genes for RT-qPCR Analysis of Lipid Biosynthesis-Related Genes inPlants (Basel, Switzerland) · 2026Article
- Integrative Physiological-Biochemical and Multi-Omics Analyses Reveal Regulatory Mechanisms Governing Growth, Selenium Accumulation, and Nutrient Metabolism in Selenium Biologically Enhanced Quinoa (Chenopodium quinoa Willd.).Journal of agricultural and food chemistry · 2026Article
- Molecular Characterization of Omega-3 Fatty Acid Desaturases Reveals Functional Conservation and Their Pivotal Role in Salt and Temperature Stress Adaptation inInternational journal of molecular sciences · 2026Article
- Integrative GWAS and transcriptomic analyses identify candidate genes associated with oil content and fatty acid composition in safflower.BMC plant biology · 2026Article
- Telomere-to-telomere genome assembly of linseed (Linum usitatissimum L.) for functional genomics and accelerated genetic improvement.Plant biotechnology journal · 2025Article
- Genome-Wide Identification ofPlants (Basel, Switzerland) · 2025Article
- Genome-wide analysis of fatty acid desaturase genes in moso bamboo (Phyllostachys edulis) reveal their important roles in abiotic stresses responses.BMC genomics · 2024Article
- Chia (Salvia hispanica L.), a functional 'superfood': new insights into its botanical, genetic and nutraceutical characteristics.Annals of botany · 2024Review
- Comparative genomics points to tandem duplications of SAD gene clusters as drivers of increased α-linolenic (ω-3) content in S. hispanica seeds.The plant genome · 2024Article
- The Genome of the Korean Island-OriginatedGenes · 2023Article
- Overexpression of the Purple Perilla (International journal of molecular sciences · 2023Article
- Physaria fendleri FAD3-1 overexpression increases ɑ-linolenic acid content in Camelina sativa seeds.Scientific reports · 2023Article
- Biochar-Dual Oxidant Composite Particles Alleviate the Oxidative Stress of Phenolic Acid on Tomato Seed Germination.Antioxidants (Basel, Switzerland) · 2023Article
- A near complete genome assembly of chia assists in identification of key fatty acid desaturases in developing seeds.Frontiers in plant science · 2023Article
- Fatty acid desaturases (FADs) modulate multiple lipid metabolism pathways to improve plant resistance.Molecular biology reports · 2022Review
- A chromosome-level genome assembly of chia provides insights into high omega-3 content and coat color variation of its seeds.Plant communications · 2022Article
- Advances in Understanding the Genetic Basis of Fatty Acids Biosynthesis in Perilla: An Update.Plants (Basel, Switzerland) · 2022Review
- Exploring the Effect of Methyl Jasmonate on the Expression of microRNAs Involved in Biosynthesis of Active Compounds of Rosemary Cell Suspension Cultures through RNA-Sequencing.International journal of molecular sciences · 2022Article
- Increase in alpha-linolenic acid content by simultaneous expression of fatty acid metabolism genes in Sesame (Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Overexpression ofGenes · 2022Article
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Abstract
Omega-3 fatty acid desaturase (ω-3 FAD, D15D) is a key enzyme for α-linolenic acid (ALA) biosynthesis. Both chia (Salvia hispanica) and perilla (Perilla frutescens) contain high levels of ALA in seeds. In this study, the ω-3 FAD gene family was systematically and comparatively cloned from chia and perilla. Perilla FAD3, FAD7, FAD8 and chia FAD7 are encoded by single-copy (but heterozygous) genes, while chia FAD3 is encoded by 2 distinct genes. Only 1 chia FAD8 sequence was isolated. In these genes, there are 1 to 6 transcription start sites, 1 to 8 poly(A) tailing sites, and 7 introns. The 5'UTRs of PfFAD8a/b contain 1 to 2 purine-stretches and 2 pyrimidine-stretches. An alternative splice variant of ShFAD7a/b comprises a 5'UTR intron. Their encoded proteins harbor an FA_desaturase conserved domain together with 4 trans-membrane helices and 3 histidine boxes. Phylogenetic analysis validated their identity of dicot microsomal or plastidial ω-3 FAD proteins, and revealed some important evolutionary features of plant ω-3 FAD genes such as convergent evolution across different phylums, single-copy status in algae, and duplication events in certain taxa. The qRT-PCR assay showed that the ω-3 FAD genes of two species were expressed at different levels in various organs, and they also responded to multiple stress treatments. The functionality of the ShFAD3 and PfFAD3 enzymes was confirmed by yeast expression. The systemic molecular and functional features of the ω-3 FAD gene family from chia and perilla revealed in this study will facilitate their use in future studies on genetic improvement of ALA traits in oilseed crops.
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