Evidence map›Paper›PMID 29351211›Full record

ArticleSensors (Basel, Switzerland)2018

Wide-Field Fluorescence Microscopy of Real-Time Bioconjugation Sensing.

Marcin Szalkowski, Karolina Sulowska, Justyna Grzelak, Joanna Niedziółka-Jönsson, Ewa Roźniecka, Dorota Kowalska, Sebastian Maćkowski

Abstract read
In one paragraph

Article in Sensors (Basel, Switzerland), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Plasmonics with Metallic Nanowires.Materials (Basel, Switzerland) · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Marcin SzalkowskiFaculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Grudziadzka 5, 87-100 Torun, Poland. marszal@fizyka.umk.pl.
Karolina SulowskaFaculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Grudziadzka 5, 87-100 Torun, Poland. sulowska@fizyka.umk.pl.
Justyna GrzelakFaculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Grudziadzka 5, 87-100 Torun, Poland. justynag@fizyka.umk.pl.
Joanna Niedziółka-JönssonInstitute of Physical Chemistry Polish Academy of Sciences, ul. Kasprzaka 44/52, 01-224 Warsaw, Poland. jniedziolka@ichf.edu.pl.
Ewa RoźnieckaInstitute of Physical Chemistry Polish Academy of Sciences, ul. Kasprzaka 44/52, 01-224 Warsaw, Poland. erozniecka@ichf.edu.pl.
Dorota KowalskaFaculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Grudziadzka 5, 87-100 Torun, Poland. dorota@fizyka.umk.pl.ORCID 0000-0002-9994-1075
Sebastian MaćkowskiFaculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Grudziadzka 5, 87-100 Torun, Poland. mackowski@fizyka.umk.pl.ORCID 0000-0003-1560-6315

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We apply wide-field fluorescence microscopy to measure real-time attachment of photosynthetic proteins to plasmonically active silver nanowires. The observation of this effect is enabled, on the one hand, by sensitive detection of fluorescence and, on the other hand, by plasmonic enhancement of protein fluorescence. We examined two sample configurations with substrates being a bare glass coverslip and a coverslip functionalized with a monolayer of streptavidin. The different preparation of the substrate changes the observed behavior as far as attachment of the protein is concerned as well as its subsequent photobleaching. For the latter substrate the conjugation process is measurably slower. The described method can be universally applied in studying protein-nanostructure interactions for real-time fluorescence-based sensing.

Indexed as

Microscopy, FluorescenceFluorescenceNanostructuresNanowiresSilverSpectrometry, FluorescenceSilverbioconjugationfluorescence imagingplasmon enhancementsilver nanowire

Identifiers

PMID29351211
PMCPMC5796375

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.