Evidence map›Paper›PMID 29350293›Full record

ArticleWorld journal of microbiology & biotechnology2018

Diversity and enzymatic potentialities of Bacillus sp. strains isolated from a polluted freshwater ecosystem in Cuba.

Jeny Adina Larrea-Murrell, Marcia Maria Rojas-Badia, Ivette García-Soto, Beatriz Romeu-Alvarez, Tristano Bacchetti, Annika Gillis, Ana Karina Boltes-Espinola, Mayra Heydrich-Perez, Daysi Lugo-Moya, Jacques Mahillon

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In one paragraph

Article in World journal of microbiology & biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.0field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 3 countries.

Jeny Adina Larrea-MurrellLaboratory of Microbial Ecology, Microbiology and Virology Department, Biology Faculty, Havana University, Havana, Cuba.
Marcia Maria Rojas-BadiaLaboratory of Microbial Ecology, Microbiology and Virology Department, Biology Faculty, Havana University, Havana, Cuba. marcia@fbio.uh.cu.
Ivette García-SotoLaboratory of Microbial Ecology, Microbiology and Virology Department, Biology Faculty, Havana University, Havana, Cuba.
Beatriz Romeu-AlvarezLaboratory of Microbial Ecology, Microbiology and Virology Department, Biology Faculty, Havana University, Havana, Cuba.
Tristano BacchettiInstituto Madrileño de Estudios Avanzados Agua (IMDEA Agua). C/Punto Net 4, Parque Científico Tecnológico de la Universidad de Alcalá, 28805, Alcalá de Henares, Madrid, Spain.
Annika GillisLaboratory of Food and Environmental Microbiology, Earth and Life Institute, Université catholique de Louvain, Louvain-la-Neuve, Belgium.
Ana Karina Boltes-EspinolaInstituto Madrileño de Estudios Avanzados Agua (IMDEA Agua). C/Punto Net 4, Parque Científico Tecnológico de la Universidad de Alcalá, 28805, Alcalá de Henares, Madrid, Spain.
Mayra Heydrich-PerezLaboratory of Microbial Ecology, Microbiology and Virology Department, Biology Faculty, Havana University, Havana, Cuba.
Daysi Lugo-MoyaLaboratory of Microbial Ecology, Microbiology and Virology Department, Biology Faculty, Havana University, Havana, Cuba.
Jacques MahillonLaboratory of Food and Environmental Microbiology, Earth and Life Institute, Université catholique de Louvain, Louvain-la-Neuve, Belgium.
University of Havana · CUUCLouvain · BEIMDEA Water · ESUniversidad de Alcalá · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Genotypic and phenotypic characterization of Bacillus spp. from polluted freshwater has been poorly addressed. The objective of this research was to determine the diversity and enzymatic potentialities of Bacillus spp. strains isolated from the Almendares River. Bacilli strains from a polluted river were characterized by considering the production of extracellular enzymes using API ZYM. 14 strains were selected and identified using 16S rRNA, gyrB and aroE genes. Genotypic diversity of the Bacillus spp. strains was evaluated using pulsed field gel electrophoresis. Furthermore, the presence of genetic determinants of potential virulence toxins of the Bacillus cereus group and proteinaceous crystal inclusions of Bacillus thuringiensis was determined. 10 strains were identified as B. thuringiensis, two as Bacillus megaterium, one as Bacillus pumilus and one as Bacillus subtilis. Most strains produced proteases, amylases, phosphatases, esterases, aminopeptidases and glucanases, which reflect the abundance of biopolymeric matter in Almendares River. Comparison of the typing results revealed a spatio-temporal distribution among B. thuringiensis strains along the river. The results of the present study highlight the genotypic and phenotypic diversity of Bacillus spp. strains from a polluted river, which contributes to the knowledge of genetic diversity of Bacilli from tropical polluted freshwater ecosystems.

Indexed as

BiodiversityEcosystemWater MicrobiologyBacillusBacterial ProteinsBacterial ToxinsCubaDNA, BacterialDNA GyraseElectrophoresis, Gel, Pulsed-FieldEnzyme AssaysFresh WaterGenes, BacterialGenotypePhylogenyRiversBacterial ProteinsBacterial ToxinsDNA, BacterialDNA GyraseRNA, Ribosomal, 16SBacillus spp.Bacillus thuringiensisEnzyme activityFreshwaterPolluted river

Identifiers

PMID29350293
OpenAlexW2793825246

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.