Evidence map›Paper›PMID 29349874›Full record

ArticleProteomics. Clinical applications2018

Assessment by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry of the Effects of Preanalytical Variables on Serum Peptidome Profiles Following Long-Term Sample Storage.

Sachio Tsuchida, Mamoru Satoh, Hiroshi Umemura, Kazuyuki Sogawa, Masaki Takiwaki, Takayuki Ishige, Yui Miyabayashi, Yuuya Iwasawa, Sohei Kobayashi, Minako Beppu and 4 more

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Article in Proteomics. Clinical applications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors.

Sachio TsuchidaDivision of Clinical Mass Spectrometry, Chiba University Hospital, Chiba, Japan.
Mamoru SatohDivision of Clinical Mass Spectrometry, Chiba University Hospital, Chiba, Japan.
Hiroshi UmemuraDepartments of Dermatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Kazuyuki SogawaDepartment of Biochemistry, School of Life and Environmental Science, Azabu University, Sagamihara, Japan.
Masaki TakiwakiDivision of Clinical Mass Spectrometry, Chiba University Hospital, Chiba, Japan.
Takayuki IshigeDepartment of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan.
Yui MiyabayashiDivision of Clinical Mass Spectrometry, Chiba University Hospital, Chiba, Japan.
Yuuya IwasawaDivision of Clinical Mass Spectrometry, Chiba University Hospital, Chiba, Japan.
Sohei KobayashiDepartment of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan.
Minako BeppuDepartment of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan.
Motoi NishimuraDepartment of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan.
Yoshio KoderaLaboratory of Biomolecular Dynamics, Department of physics, School of Science, Kitasato University, Chiba, Japan.
Kazuyuki MatsushitaDepartment of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan.
Fumio NomuraDivision of Clinical Mass Spectrometry, Chiba University Hospital, Chiba, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeHuman serum and plasma are often used as clinical specimens in proteomics analyses, and peptidome profiling of human serum is a promising tool for identifying novel disease-associated biomarkers. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is widely used for peptidomic biomarker discovery. Careful sample collection and handling are required as either can have a profound impact on serum peptidome patterns, yet the effects of preanalytical variables on serum peptidome profiles have not been completely elucidated. The present study investigated the effects of preanalytical variables, including storage temperature, duration (up to 12 months), and thawing methods, on MALDI-TOF MS-based serum peptidome patterns. EXPERIMENTAL

designAliquots of serum samples were pretreated with weak cation exchanger magnetic beads using an automated ClinProtRobot system and then analyzed by MALDI-TOF MS.

resultsA number of significant differences in peak intensities were observed depending on sample processing variables. CONCLUSIONS AND CLINICAL RELEVANCE: These peaks can be used as sample quality markers to assess the effects of long-term storage on serum peptidome profiles using MALDI-TOF MS.

Indexed as

Specimen HandlingAdultFemaleHumansMalePeptidesProteomicsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTime FactorsPeptidesClinProtRobotlong-term sample storageMALDI-TOF MSserum peptidome

Identifiers

PMID29349874
PMCPMC5947747

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.