ArticleScientific reports2018
Characterization of a Novel Tectivirus Phage Toil and Its Potential as an Agent for Biolipid Extraction.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Article
- Expanding the Diversity ofViruses · 2025Article
- Transcriptional dynamics during Rhodococcus erythropolis infection with phage WC1.BMC microbiology · 2024Article
- Review
- Comparative Genomics of Prophages Sato and Sole Expands the Genetic Diversity Found in the GenusMicroorganisms · 2021Article
- Dual-function oleaginous biocatalysts for non-sterile cultivation and solvent-free biolipid bioextraction to reduce biolipid-based biofuel production costs.The Science of the total environment · 2021Article
- Actinobacteriophages: Genomics, Dynamics, and Applications.Annual review of virology · 2020Review
- A Novel Genus of Actinobacterial Tectiviridae.Viruses · 2019Article
- Half a Century of Research on Membrane-Containing Bacteriophages: Bringing New Concepts to Modern Virology.Viruses · 2019Review
- Development ofBiotechnology for biofuels · 2019Review
- Simultaneous lipid biosynthesis and recovery for oleaginous yeastBiotechnology for biofuels · 2019Article
- Current taxonomy of Rhodococcus species and their role in infections.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Review
- Lytic gene expression in the temperate bacteriophage GIL01 is activated by a phage-encoded LexA homologue.Nucleic acids research · 2018Article
- Vast diversity of prokaryotic virus genomes encoding double jelly-roll major capsid proteins uncovered by genomic and metagenomic sequence analysis.Virology journal · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The oleaginous bacterium Rhodococcus opacus PD630 is metabolically diverse and can be cultivated on various renewable resources to serve as a sustainable triacylglycerol (TAG) feedstock for biodiesel production. Current methods for TAG extraction are costly, but infection of cultures by lytic bacteriophages (phages) may be a viable approach for achieving release of intracellular lipid from oleaginous bacteria such as R. opacus. This study reports the novel tectiviral phage Toil capable of releasing intracellular contents including a fluorescent protein marker and TAGs into the supernatant after phage infection of R. opacus PD631, a domesticated derivative of strain PD630. Phage Toil is placed in the Tectiviridae by its morphology, the presence of a lipid membrane, its genome architecture and the presence of terminal covalently-linked proteins. Toil is the first tectivirus capable of infecting a member of the Actinobacteria. Microscopy shows that infected cells do not undergo sudden lysis but instead maintain their original shape for several hours, with the cellular morphology gradually deteriorating. Approximately 30% of intracellular TAGs could be recovered from the culture supernatants of Toil-infected PD631 cells. Phage Toil has potential to be used as an agent in extraction of TAGs from oleaginous bacterium R. opacus. IMPORTANCE: This study reported the first tectivirus (Phage Toil) capable of infecting a member of the Actinobacteria. In this study, we showed that Phage Toil can infect oleaginous bacterium Rhodococcus opacus to release intracellular contents such as a fluorescent protein marker and TAG lipid granules, which can serve as a starting material for biodiesel production. This study demonstrates a new method to extract TAGs by using this phage. Additionally, Phage Toil can be a new model phage to advance knowledge regarding phage infection mechanisms in Rhodococcus and other mycolic acid-containing bacteria such as Mycobacterium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.