ArticleParasites & vectors2018
Establishment of an in vitro chicken epithelial cell line model to investigate Eimeria tenella gamete development.
Article in Parasites & vectors, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 47 citations in OpenAlex.
- An oral Eimeria-vectored vaccine induces protective immunity against chicken infectious anemia.NPJ vaccines · 2026Article
- Bioluminescence-basedFrontiers in cellular and infection microbiology · 2026Article
- Novel organoids andParasitology · 2025Review
- Cultivating the endogenous life cycle ofParasitology · 2025Article
- Genetic manipulation for the non-model protozoaniScience · 2025Review
- Potential Development Ability of Residual Zoites, a Second-Generation Meront, Inducing Long-Term Infection by the Mouse Eimerian Parasite, Eimeria krijgsmanni.Acta parasitologica · 2024Article
- Histopathologic observations in a coccidiosis model of Eimeria tenella.Animal models and experimental medicine · 2024Article
- Eimeria: Navigating complex intestinal ecosystems.PLoS pathogens · 2024Review
- Mycotoxins and coccidiosis in poultry - co-occurrence, interaction, and effects.Frontiers in veterinary science · 2024Review
- Article
- Natural Magnolol ameliorates coccidiosis infected with Eimeria tenella by affecting antioxidant, anti-inflammatory, and gut microbiota of chicks.Poultry science · 2023Article
- Overexpression of Eimeria tenella Rhoptry Kinase 2 Induces Early Production of Schizonts.Microbiology spectrum · 2023Article
- The in vitro and in vivo anti-virulent effect of organic acid mixtures against Eimeria tenella and Eimeria bovis.Scientific reports · 2021Article
- In vitro infection of Madin-Darby bovine kidney (MDBK) cells with Eimeria acervulina sporozoites: quantitative analysis of parasite cellular invasion and replication using real-time polymerase chain reaction (PCR).Parasitology research · 2021Article
- In Vitro Assessment of Anticoccidials: Methods and Molecules.Animals : an open access journal from MDPI · 2021Review
- Dual RNA-Seq transcriptome analysis of chicken macrophage-like cells (HD11) infectedParasitology · 2021Article
- Inside-out chicken enteroids with leukocyte component as a model to study host-pathogen interactions.Communications biology · 2021Article
- Evaluation of 4 merozoite antigens as candidate vaccines against Eimeria tenella infection.Poultry science · 2021Article
- Isolation, culture, and characterization of chicken intestinal epithelial cells.BMC molecular and cell biology · 2021Article
- A Novel Rhoptry Protein as Candidate Vaccine againstVaccines · 2020Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEimeria tenella infection leads to acute intestinal disorders responsible for important economic losses in poultry farming worldwide. The life-cycle of E. tenella is monoxenous with the chicken as the exclusive host; infection occurs in caecal epithelial cells. However, in vitro, the complete life-cycle of the parasite has only been propagated successfully in primary chicken kidney cells, which comprise undefined mixed cell populations; no cell line model has been able to consistently support the development of the sexual stages of the parasite. We therefore sought to develop a new model to study E. tenella gametogony in vitro using a recently characterised chicken cell line (CLEC-213) exhibiting an epithelial cell phenotype.
methodsCLEC-213 were infected with sporozoites from a precocious strain or with second generation merozoites (merozoites II) from wild type strains. Sexual stages of the parasite were determined both at the gene and protein levels.
resultsTo our knowledge, we show for the first time in CLEC-213, that sporozoites from a precocious strain of E. tenella were able to develop to gametes, as verified by measuring gene expression and by using antibodies to a microgamete-specific protein (EtFOA1: flagellar outer arm protein 1) and a macrogamete-specific protein (EtGAM-56), but oocysts were not observed. However, both gametes and oocysts were observed when cells were infected with merozoites II from wild type strains, demonstrating that completion of the final steps of the parasite cycle is possible in CLEC-213 cells.
conclusionThe epithelial cell line CLEC-213 constitutes a useful avian tool for studying Eimeria epithelial cell interactions and the effect of drugs on E. tenella invasion, merogony and gametogony.
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