Evidence map›Paper›PMID 29345326›Full record

ArticleCurrent protocols in cytometry2018

Live-Animal Imaging of Renal Function by Multiphoton Microscopy.

Kenneth W Dunn, Timothy A Sutton, Ruben M Sandoval

Abstract read
In one paragraph

Article in Current protocols in cytometry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
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  11. Article
  12. The osteocyte as a signaling cell.Physiological reviews · 2022
    Review
  13. Article
  14. Review
  15. The Indiana O'Brien Center for Advanced Renal Microscopic Analysis.American journal of physiology. Renal physiology · 2021
    Review
  16. Review
  17. Intravital Imaging Techniques for Biomedical and Clinical Research.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2020
    Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Kenneth W DunnIndiana University School of Medicine, Indianapolis, Indiana.
Timothy A SuttonIndiana University School of Medicine, Indianapolis, Indiana.
Ruben M SandovalIndiana University School of Medicine, Indianapolis, Indiana.

Funding

Translation CoreP30DK097512 · NIDDK · INDIANA UNIVERSITY INDIANAPOLIS · PI Carmella Evans-Molina · 2015 to 2026
$17.4M
PROBE DELIVERY COREP30DK079312 · NIDDK · INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS · PI MOLITORIS, BRUCE A · 2007 to 2021
$17.1M
Proximal Tubule Albumin Transport in Disease States.R01DK091623 · NIDDK · INDIANA UNIVERSITY INDIANAPOLIS · PI MOLITORIS, BRUCE A · 2011 to 2021
$4.3M
Modifying kidney injury through p53 signaling.R01DK099345 · NIDDK · INDIANA UNIVERSITY INDIANAPOLIS · PI DAGHER, PIERRE C, SUTTON, TIMOTHY ALAN · 2014 to 2017
$1.4M
NIDDK NIH HHS P30 DK079312NIDDK NIH HHS P30 DK097512NIDDK NIH HHS R01 DK091623NIDDK NIH HHS R01 DK099345
6 · The paper itself

Abstract

Intravital microscopy, microscopy of living animals, is a powerful research technique that combines the resolution and sensitivity found in microscopic studies of cultured cells with the relevance and systemic influences of cells in the context of the intact animal. The power of intravital microscopy has recently been extended with the development of multiphoton fluorescence microscopy systems capable of collecting optical sections from deep within the kidney at subcellular resolution, supporting high-resolution characterizations of the structure and function of glomeruli, tubules, and vasculature in the living kidney. Fluorescent probes are administered to an anesthetized, surgically prepared animal, followed by image acquisition for up to 3 hr. Images are transferred via a high-speed network to specialized computer systems for digital image analysis. This general approach can be used with different combinations of fluorescent probes to evaluate processes such as glomerular permeability, proximal tubule endocytosis, microvascular flow, vascular permeability, mitochondrial function, and cellular apoptosis/necrosis. © 2018 by John Wiley & Sons, Inc.

Indexed as

AnimalsFluorescent DyesKidneyMicroscopy, Fluorescence, MultiphotonMicroscopy, VideoRatsRats, WistarFluorescent Dyesfluorescence microscopyintravital microscopyin vivo microscopymultiphoton microscopy

Identifiers

PMID29345326
PMCPMC5774340

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.