ArticleAmerican journal of physiology. Lung cellular and molecular physiology2018
Transient stretch induces cytoskeletal fluidization through the severing action of cofilin.
Article in American journal of physiology. Lung cellular and molecular physiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Vascular Smooth Muscle Myosin 2 Filaments Dynamically Assemble and Stabilize During Induced Contractility.Arteriosclerosis, thrombosis, and vascular biology · 2026Article
- An Opto-Actuated Hydrogel for Cell Mechanoactuation and Real-Time Force Monitoring.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Actin cytoskeleton protection by the formin-mediated safety valve.Current opinion in cell biology · 2025Review
- Adherent cells undergo rate softening mediated by actomyosin kinetics.Biophysical journal · 2025Article
- A life off the beaten track in biomechanics: Imperfect elasticity, cytoskeletal glassiness, and epithelial unjamming.Biophysics reviews · 2023Review
- The effect of cyclic stretch on aortic viscoelasticity and the putative role of smooth muscle focal adhesion.Frontiers in physiology · 2023Article
- Intrinsic cell rheology drives junction maturation.Nature communications · 2022Article
- Vimentin intermediate filaments and filamentous actin form unexpected interpenetrating networks that redefine the cell cortex.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Are cell jamming and unjamming essential in tissue development?Cells & development · 2021Review
- Single Cell Forces after Electroporation.ACS nano · 2021Article
- Structural and mechanical remodeling of the cytoskeleton maintains tensional homeostasis in 3D microtissues under acute dynamic stretch.Scientific reports · 2020Article
- Mechanobiology of dynamic enzyme systems.APL bioengineering · 2020Article
- Tissue traction microscopy to quantify muscle contraction within precision-cut lung slices.American journal of physiology. Lung cellular and molecular physiology · 2020Article
- Airway smooth muscle tone increases actin filamentogenesis and contractile capacity.American journal of physiology. Lung cellular and molecular physiology · 2020Article
- Inhibiting Airway Smooth Muscle Contraction Using Pitavastatin: A Role for the Mevalonate Pathway in Regulating Cytoskeletal Proteins.Frontiers in pharmacology · 2020Article
- The Aftermath of Bronchoconstriction.Journal of engineering and science in medical diagnostics and therapy · 2019Review
- Mitigation of airways responsiveness by deep inflation of the lung.Journal of applied physiology (Bethesda, Md. : 1985) · 2018Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
With every deep inspiration (DI) or sigh, the airway wall stretches, as do the airway smooth muscle cells in the airway wall. In response, the airway smooth muscle cell undergoes rapid stretch-induced cytoskeletal fluidization. As a molecular mechanism underlying the cytoskeletal fluidization response, we demonstrate a key role for the actin-severing protein cofilin. Using primary human airway smooth muscle cells, we simulated a DI by imposing a transient stretch of physiological magnitude and duration. We used traction microscopy to measure the resulting changes in contractile forces. After a transient stretch, cofilin-knockdown cells exhibited a 29 ± 5% decrease in contractile force compared with prestretch conditions. By contrast, control cells exhibited a 67 ± 6% decrease ( P < 0.05, knockdown vs. control). Consistent with these contractile force changes with transient stretch, actin filaments in cofilin-knockdown cells remained largely intact, whereas actin filaments in control cells were rapidly disrupted. Furthermore, in cofilin-knockdown cells, contractile force at baseline was higher and rate of remodeling poststretch was slower than in control cells. Additionally, the severing action of cofilin was restricted to the release phase of the transient stretch. We conclude that the actin-severing activity of cofilin is an important factor in stretch-induced cytoskeletal fluidization and may account for an appreciable part of the bronchodilatory effects of a DI.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.