ArticleMethods in molecular biology (Clifton, N.J.)2018
Laser-Capture Microdissection and RNA Extraction from Perfusion-Fixed Cartilage and Bone Tissue from Mice Implanted with Human iPSC-Derived MSCs in a Calvarial Defect Model.
Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 5 citations in OpenAlex.
- Spatial transcriptomic profiling of decalcified murine musculoskeletal samples via Xenium Prime 5K.JBMR plus · 2026Article
- Spatial transcriptomic applications in orthopedics.Connective tissue research · 2025Review
- Extracting high-quality RNA from formaldehyde-fixed naturally aged neuromusculoskeletal tissues.BioTechniques · 2024Article
- Effect of two (short-term) storage methods on load to failure testing of murine bone tissue.Scientific reports · 2019Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Laser-capture microdissection (LCM) coupled to downstream RNA analysis poses unique difficulties for the evaluation of mineralized tissues. A rapid protocol was thus developed to enable sufficient integrity of bone and cartilage tissue for reliable sectioning, while minimizing RNA loss associated with prolonged decalcification and purification steps. Specifically, the protocol involves pump-assisted, cardiac perfusion-fixation with paraformaldehyde, and moderate digestion of LCM-acquired tissue with proteinase K followed by DNase treatment and separation of RNA using magnetic beads. Reverse transcription and cDNA synthesis are performed immediately after RNA purification, without need for further protein removal.
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Registered trials
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