ArticleScientific reports2018
Counting Caenorhabditis elegans: Protocol Optimization and Applications for Population Growth and Toxicity Studies in Liquid Medium.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Article
- Toxicity of Four Common Environmental Chemicals Across Caenorhabditis elegans Life Stages Supporting the One Health Concept.Environmental toxicology · 2026Article
- The Importance of Biotinylation for the Suitability of Cationic and Neutral Fourth-Generation Polyamidoamine Dendrimers as Targeted Drug Carriers in the Therapy of Glioma and Liver Cancer.Molecules (Basel, Switzerland) · 2024Article
- Repurposed Drugs Celecoxib and Fmoc-L-Leucine Alone and in Combination as Temozolomide-Resistant Antiglioma Agents-Comparative Studies on Normal and Immortalized Cell Lines, and onInternational journal of molecular sciences · 2024Article
- Assessment of the effects of cannabidiol and a CBD-rich hemp extract inFrontiers in toxicology · 2024Article
- Microbes are potential key players in the evolution of life histories and aging inEcology and evolution · 2023Article
- Exploring the Potential of Lapatinib, Fulvestrant, and Paclitaxel Conjugated with Glycidylated PAMAM G4 Dendrimers for Cancer and Parasite Treatment.Molecules (Basel, Switzerland) · 2023Article
- Evidence for Complex Interplay between Quorum Sensing and Antibiotic Resistance in Pseudomonas aeruginosa.Microbiology spectrum · 2022Article
- Article
- The Bacillary Postbiotics, Including 2-Undecanone, Suppress the Virulence of Pathogenic Microorganisms.Pharmaceutics · 2022Article
- Biotin Transport-Targeting Polysaccharide-Modified PAMAM G3 Dendrimer as System Delivering α-Mangostin into Cancer Cells andInternational journal of molecular sciences · 2021Article
- Investigation of anthelmintic activity of the acetone extract and constituents of Typha capensis against animal parasitic Haemonchus contortus and free-living Caenorhabditis elegans.Parasitology research · 2021Article
- Risperidone induced alterations in feeding and locomotion behavior ofCurrent research in toxicology · 2021Article
- Molecular and cellular responses to short exposure to bisphenols A, F, and S and eluates of microplastics in C. elegans.Environmental science and pollution research international · 2021Article
- A quantitative medium-throughput assay to measureSTAR protocols · 2020Article
- A robust and miniaturized screening platform to study natural products affecting metabolism and survival in Caenorhabditis elegans.Scientific reports · 2020Article
- Emerging Standards and Analytical Science for Nanoenabled Medical Products.Annual review of analytical chemistry (Palo Alto, Calif.) · 2020Review
- Oxygen consumption rate of Caenorhabditis elegans as a high-throughput endpoint of toxicity testing using the Seahorse XFScientific reports · 2020Article
- Review
- Neonicotinoid-containing insecticide disruption of growth, locomotion, and fertility in Caenorhabditis elegans.PloS one · 2020Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nematode Caenorhabditis elegans is used extensively in molecular, toxicological and genetics research. However, standardized methods for counting nematodes in liquid culture do not exist despite the wide use of nematodes and need for accurate measurements. Herein, we provide a simple and affordable counting protocol developed to maximize count accuracy and minimize variability in liquid nematode culture. Sources of variability in the counting process were identified and tested in 14 separate experiments. Three variables resulted in significant effects on nematode count: shaking of the culture, priming of pipette tips, and sampling location within a microcentrifuge tube. Between-operator variability did not have a statistically significant effect on counts, even among differently-skilled operators. The protocol was used to assess population growth rates of nematodes in two different but common liquid growth media: axenic modified Caenorhabditis elegans Habitation and Reproduction medium (mCeHR) and S-basal complete. In mCeHR, nematode populations doubled daily for 10 d. S-basal complete populations initially doubled every 12 h, but slowed within 7 d. We also detected a statistically significant difference between embryo-to-hatchling incubation period of 5 d in mCeHR compared to 4 d in S-basal complete. The developed counting method for Caenorhabditis elegans reduces variability and allows for rigorous and reliable experimentation.
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