ArticleMolecular biology of the cell2018
Constitutive centromere-associated network contacts confer differential stability on CENP-A nucleosomes in vitro and in the cell.
Article in Molecular biology of the cell, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Phosphorylation of Xenopus M18BP1 governs centromeric localization and CENP-A nucleosome assembly.EMBO reports · 2026Article
- Histone H3 lysine methyltransferase activities control compartmentalization of human centromeres.bioRxiv : the preprint server for biology · 2025Article
- Independence of centromeric and pericentromeric chromatin stability on CCAN components.Molecular biology of the cell · 2025Article
- Vertebrate centromere architecture: from chromatin threads to functional structures.Chromosoma · 2024Review
- Centromere Chromatin Dynamics at a Glance.Epigenomes · 2022Review
- The Mis6 inner kinetochore subcomplex maintains CENP-A nucleosomes against centromeric non-coding transcription during mitosis.Communications biology · 2022Article
- DiMeLo-seq: a long-read, single-molecule method for mapping protein-DNA interactions genome wide.Nature methods · 2022Article
- Structure of the human inner kinetochore bound to a centromeric CENP-A nucleosome.Science (New York, N.Y.) · 2022Article
- CENP-N promotes the compaction of centromeric chromatin.Nature structural & molecular biology · 2022Article
- Hi-CO: 3D genome structure analysis with nucleosome resolution.Nature protocols · 2021Article
- Assembly principles and stoichiometry of a complete human kinetochore module.Science advances · 2021Article
- Stable inheritance of CENP-A chromatin: Inner strength versus dynamic control.The Journal of cell biology · 2020Article
- Meiotic CENP-C is a shepherd: bridging the space between the centromere and the kinetochore in time and space.Essays in biochemistry · 2020Review
- CENP-A nucleosome-a chromatin-embedded pedestal for the centromere: lessons learned from structural biology.Essays in biochemistry · 2020Review
- Cryoelectron Microscopy Structure of a Yeast Centromeric Nucleosome at 2.7 Å Resolution.Structure (London, England : 1993) · 2020Article
- CDK1-mediated CENP-C phosphorylation modulates CENP-A binding and mitotic kinetochore localization.The Journal of cell biology · 2019Article
- Quiescent Cells Actively Replenish CENP-A Nucleosomes to Maintain Centromere Identity and Proliferative Potential.Developmental cell · 2019Article
- An improved auxin-inducible degron system preserves native protein levels and enables rapid and specific protein depletion.Genes & development · 2019Article
- De novo formation and epigenetic maintenance of centromere chromatin.Current opinion in cell biology · 2019Review
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Authors and funding
5 authors at 4 institutions in 1 country.
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Abstract
Eukaryotic centromeres are defined by the presence of nucleosomes containing the histone H3 variant, centromere protein A (CENP-A). Once incorporated at centromeres, CENP-A nucleosomes are remarkably stable, exhibiting no detectable loss or exchange over many cell cycles. It is currently unclear whether this stability is an intrinsic property of CENP-A containing chromatin or whether it arises from proteins that specifically associate with CENP-A chromatin. Two proteins, CENP-C and CENP-N, are known to bind CENP-A human nucleosomes directly. Here we test the hypothesis that CENP-C or CENP-N stabilize CENP-A nucleosomes in vitro and in living cells. We show that CENP-N stabilizes CENP-A nucleosomes alone and additively with CENP-C in vitro. However, removal of CENP-C and CENP-N from cells, or mutating CENP-A so that it no longer interacts with CENP-C or CENP-N, had no effect on centromeric CENP-A stability in vivo. Thus, the stability of CENP-A nucleosomes in chromatin does not arise solely from its interactions with CENP-C or CENP-N.
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Registered trials
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