ReviewThe Plant cell2018
Revisiting Criteria for Plant MicroRNA Annotation in the Era of Big Data.
Review in The Plant cell, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 219 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
219 citing papers in PubMed, 1 synthesis or guideline pooled it, 446 citations in OpenAlex.
- The role of microRNAs in responses to drought and heat stress in peanut (Arachis hypogaea).The plant genome · 2023Pooled it
- Systematic discovery of immunomodulatory plant-derived nanoparticles reveals RNA-mediated macrophage reprogramming.Bioactive materials · 2026Article
- A microRNA Catalogue for Red Abalone Larvae Reveals Developmental and Acidification-Associated Abundance Patterns.Marine biotechnology (New York, N.Y.) · 2026Article
- Article
- Integrative RNA sequencing uncovers the miRNA-mRNA landscape of water-deficit response in Setaria italica.Planta · 2026Article
- Proteomic and Small RNA Characterization of Extracellular Vesicle-enriched Particles Released from Cultured Host-isolated Symbiodiniaceae.Marine biotechnology (New York, N.Y.) · 2026Article
- Article
- miR408-5p and miR408-3p cooperatively reduce cadmium uptake and accumulation in rice.PLoS biology · 2026Article
- Characterization ofInternational journal of molecular sciences · 2026Article
- New molecular players: siRNA expression and gene regulation in Hordeum vulgare L. ageing seeds after germination.BMC genomics · 2026Article
- The annotated blueprint: integrated functional genomic resources for a model tetraploid wheat Triticum turgidum cv Kronos.The New phytologist · 2026Article
- Small RNAs as systemic signals in plant defense: mechanisms, challenges, and future directions.Molecular biology reports · 2026Review
- Identification and analysis of microRNAs responsible for brown planthopper resistance in BPH14 and BPH15 pyramiding rice.BMC plant biology · 2026Article
- Genome-Wide Identification of miRNAs and Pollen-Associated miRNA-Target Relationships inPlants (Basel, Switzerland) · 2026Article
- Limitations of Single Prediction Tools in miRNA Profiling of Grapevine Viral Coinfection.Genes · 2026Article
- Integrated RNA-seq and small RNA-seq profiling reveals tissue-associated transcriptional programs and candidate miRNA-mRNA relationships across tea floral tissues.Frontiers in plant science · 2026Article
- Application of Artificial Intelligence Technology in Plant MicroRNA Research: Progress, Challenges, and Prospects.International journal of molecular sciences · 2025Review
- Integrative analysis of small RNA and degradome sequencing reveals the role of miRNAs in monoterpene biosynthesis in linalool-type Cinnamomum camphora.BMC plant biology · 2025Article
- miRNA-Mediated Regulation ofInternational journal of molecular sciences · 2025Article
- Distinct haplotypes and reversed dominance at a single-gene balanced polymorphism controlling heterodichogamy in two genera of wingnuts.Current biology : CB · 2025Article
159 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are ∼21-nucleotide-long regulatory RNAs that arise from endonucleolytic processing of hairpin precursors. Many function as essential posttranscriptional regulators of target mRNAs and long noncoding RNAs. Alongside miRNAs, plants also produce large numbers of short interfering RNAs (siRNAs), which are distinguished from miRNAs primarily by their biogenesis (typically processed from long double-stranded RNA instead of single-stranded hairpins) and functions (typically via roles in transcriptional regulation instead of posttranscriptional regulation). Next-generation DNA sequencing methods have yielded extensive data sets of plant small RNAs, resulting in many miRNA annotations. However, it has become clear that many miRNA annotations are questionable. The sheer number of endogenous siRNAs compared with miRNAs has been a major factor in the erroneous annotation of siRNAs as miRNAs. Here, we provide updated criteria for the confident annotation of plant miRNAs, suitable for the era of "big data" from DNA sequencing. The updated criteria emphasize replication and the minimization of false positives, and they require next-generation sequencing of small RNAs. We argue that improved annotation systems are needed for miRNAs and all other classes of plant small RNAs. Finally, to illustrate the complexities of miRNA and siRNA annotation, we review the evolution and functions of miRNAs and siRNAs in plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.