ArticleMetabolites2018
Impact of Prolonged Blood Incubation and Extended Serum Storage at Room Temperature on the Human Serum Metabolome.
Article in Metabolites, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.
- Pooled it
- Disease and Medication Context Shape Ex Vivo Metabolite Stability: A Pilot Study in Systemic Lupus Erythematosus.Metabolites · 2025Article
- Investigation into temporal changes in the human bloodstain lipidome.International journal of legal medicine · 2025Article
- Substantial loss of T cells upon lymphocyte isolation from heparin-anticoagulated peripheral blood.Frontiers in immunology · 2025Article
- Sample Preparation for Metabolomic Analysis in Exercise Physiology.Biomolecules · 2024Review
- Extraction Methods for Brain Biopsy NMR Metabolomics: Balancing Metabolite Stability and Protein Precipitation.Metabolites · 2024Article
- The Influence of Preanalytical Biospecimen Handling on the Measurement of B Vitamers, Amino Acids, and Other Metabolites in Blood.Biopreservation and biobanking · 2023Article
- Article
- Variability of human fasted venous plasma metabolomic profiles with tourniquet induced hemostasis.Scientific reports · 2021Article
- Metabolite Ratios as Quality Indicators for Pre-Analytical Variation in Serum and EDTA Plasma.Metabolites · 2021Article
- Lipid Metabolite Biomarkers in Cardiovascular Disease: Discovery and Biomechanism Translation from Human Studies.Metabolites · 2021Review
- Recommendations and Best Practices for Standardizing the Pre-Analytical Processing of Blood and Urine Samples in Metabolomics.Metabolites · 2020Review
- Advancing Biomarker Development Through Convergent Engagement: Summary Report of the 2nd International Danube Symposium on Biomarker Development, Molecular Imaging and Applied Diagnostics; March 14-16, 2018; Vienna, Austria.Molecular imaging and biology · 2020Article
- Temperature and time-dependent effects of delayed blood processing on oxylipin concentrations in human plasma.Prostaglandins, leukotrienes, and essential fatty acids · 2019Article
- Pre-Analytical Factors that Affect Metabolite Stability in Human Urine, Plasma, and Serum: A Review.Metabolites · 2019Review
- Article
- Effects of Long-Term Storage at -80 °C on the Human Plasma Metabolome.Metabolites · 2019Article
- Minimal variation of the plasma lipidome after delayed processing of neonatal cord blood.Metabolomics : Official journal of the Metabolomic Society · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolomics is a powerful technology with broad applications in life science that, like other -omics approaches, requires high-quality samples to achieve reliable results and ensure reproducibility. Therefore, along with quality assurance, methods to assess sample quality regarding pre-analytical confounders are urgently needed. In this study, we analyzed the response of the human serum metabolome to pre-analytical variations comprising prolonged blood incubation and extended serum storage at room temperature by using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) -based metabolomics. We found that the prolonged incubation of blood results in a statistically significant 20% increase and 4% decrease of 225 tested serum metabolites. Extended serum storage affected 21% of the analyzed metabolites (14% increased, 7% decreased). Amino acids and nucleobases showed the highest percentage of changed metabolites in both confounding conditions, whereas lipids were remarkably stable. Interestingly, the amounts of taurine and
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.