Evidence map›Paper›PMID 29341593›Full record

ArticleBiochemistry2018

Analysis of Cellular Tyrosine Phosphorylation via Chemical Rescue of Conditionally Active Abl Kinase.

Zhihong Wang, Min-Sik Kim, Isabel Martinez-Ferrando, Anthony J Koleske, Akhilesh Pandey, Philip A Cole

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Zhihong WangDepartment of Chemistry & Biochemistry, University of the Sciences , Philadelphia, Pennsylvania 19104, United States.ORCID 0000-0003-1667-3536
Min-Sik KimGlobal Center for Pharmaceutical Ingredient Materials, Department of Applied Chemistry, Kyung Hee University , Yongin, Gyeonggi, Republic of Korea.
Isabel Martinez-FerrandoDepartment of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine , Baltimore, Maryland 21205, United States.
Anthony J KoleskeDepartments of Molecular Biophysics and Biochemistry and Neuroscience, Yale University , New Haven, Connecticut 06520, United States.
Akhilesh PandeyDepartments of Oncology and Biological Chemistry, Johns Hopkins University School of Medicine , Baltimore, Maryland 21205, United States.ORCID 0000-0001-9943-6127
Philip A ColeDepartment of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine , Baltimore, Maryland 21205, United States.

Funding

Chemical Approaches to Protein PhosphorylationR01CA074305 · NCI · JOHNS HOPKINS UNIVERSITY · PI COLE, PHILIP A · 2002 to 2025
$8.0M
Laminin control of CNS dendrite and dendritic spine developmentR01NS089662 · NINDS · YALE UNIVERSITY · PI GRUTZENDLER, JAIME, KOLESKE, ANTHONY J · 2014 to 2018
$1.8M
NCI NIH HHS R01 CA074305NINDS NIH HHS R01 NS089662
6 · The paper itself

Abstract

Identifying direct substrates targeted by protein kinases is important in understanding cellular physiology and intracellular signal transduction. Mass spectrometry-based quantitative proteomics provides a powerful tool for comprehensively characterizing the downstream substrates of protein kinases. This approach is efficiently applied to receptor kinases that can be precisely, directly, and rapidly activated by some agent, such as a growth factor. However, nonreceptor tyrosine kinase Abl lacks the experimental advantage of extracellular growth factors as immediate and direct stimuli. To circumvent this limitation, we combine a chemical rescue approach with quantitative phosphoproteomics to identify targets of Abl and their phosphorylation sites with enhanced temporal resolution. Both known and novel putative substrates are identified, presenting opportunities for studying unanticipated functions of Abl under physiological and pathological conditions.

Indexed as

3T3 CellsAnimalsFusion Proteins, bcr-ablGene OntologyHEK293 CellsHumansMicePhosphorylationPoint MutationProteomicsProto-Oncogene Proteins c-ablTyrosineFusion Proteins, bcr-ablProto-Oncogene Proteins c-ablTyrosine

Identifiers

PMID29341593
PMCPMC5906802

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.