ArticleOncotarget2017
CRISPR/Cas9-mediated reversibly immortalized mouse bone marrow stromal stem cells (BMSCs) retain multipotent features of mesenchymal stem cells (MSCs).
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Article in Oncotarget, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 1 of them a synthesis that pooled it.
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49citing papers in PubMed, 1 pooled it
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49 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Meta-analysis of the Mesenchymal Stem Cells Immortalization Protocols: A Guideline for Regenerative Medicine.Current stem cell research & therapy · 2024Pooled it
- Review
- Application Strategies of Bone Marrow Mesenchymal Stromal Cells in Bone-Related Diseases.Cell proliferation · 2026Review
- Deep Learning for Predicting Spheroid Viability: Novel Convolutional Neural Network Model for Automating Quality Control for Three-Dimensional Bioprinting.Bioengineering (Basel, Switzerland) · 2025Article
- Immortalization of Mesenchymal Stem Cells for Application in Regenerative Medicine and Their Potential Risks of Tumorigenesis.International journal of molecular sciences · 2024Review
- Establishment of immortalized rabbit bone marrow mesenchymal stem cells and a preliminary study of their osteogenic differentiation capability.Animal models and experimental medicine · 2024Article
- GAPDH suppresses adenovirus-induced oxidative stress and enables a superfast production of recombinant adenovirus.Genes & diseases · 2024Article
- Establishment and characterization of a rat model of scalp-cranial composite defect for multilayered tissue engineering.Research square · 2024Article
- Adipose-derived mesenchymal stem cells (MSCs) are a superior cell source for bone tissue engineering.Bioactive materials · 2024Article
- Cell Immortality:Life (Basel, Switzerland) · 2024Review
- The other side of the coin: mesenchymal stromal cell immortalization beyond evasion of senescence.Human cell · 2023Review
- BMP4 upregulates glycogen synthesis through the SMAD/SLC2A1 (GLUT1) signaling axis in hepatocellular carcinoma (HCC) cells.Cancer & metabolism · 2023Article
- Niclosamide (NA) overcomes cisplatin resistance in human ovarian cancer.Genes & diseases · 2023Article
- Immortalization Reversibility in the Context of Cell Therapy Biosafety.International journal of molecular sciences · 2023Review
- Modeling lung diseases using reversibly immortalized mouse pulmonary alveolar type 2 cells (imPAC2).Cell & bioscience · 2022Article
- Reversibly immortalized keratinocytes (iKera) facilitate re-epithelization and skin wound healing: Potential applications in cell-based skin tissue engineering.Bioactive materials · 2022Article
- New Perspectives to Improve Mesenchymal Stem Cell Therapies for Drug-Induced Liver Injury.International journal of molecular sciences · 2022Review
- Regenerative Medicine Application of Mesenchymal Stem Cells.Advances in experimental medicine and biology · 2022Article
- A one-step construction of adenovirus (OSCA) system using the Gibson DNA Assembly technology.Molecular therapy oncolytics · 2021Article
- Establishment of an Efficient Immortalization Strategy Using HMEJ-Based bInternational journal of molecular sciences · 2021Article
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Authors and funding
32 authors.
Xue HuDepartments of Blood Transfusion, Nephrology, Orthopaedic Surgery, and General Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Li LiMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Xinyi YuDepartments of Blood Transfusion, Nephrology, Orthopaedic Surgery, and General Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Ruyi ZhangMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Shujuan YanMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Zongyue ZengMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Yi ShuMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Chen ZhaoDepartments of Blood Transfusion, Nephrology, Orthopaedic Surgery, and General Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Xingye WuDepartments of Blood Transfusion, Nephrology, Orthopaedic Surgery, and General Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Jiayan LeiDepartments of Blood Transfusion, Nephrology, Orthopaedic Surgery, and General Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Yasha LiMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Wenwen ZhangMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Chao YangMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Ke WuMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Ying WuMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Liping AnMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Shifeng HuangDepartments of Blood Transfusion, Nephrology, Orthopaedic Surgery, and General Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Xiaojuan JiMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Cheng GongMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Chengfu YuanMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Linghuan ZhangMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Wei LiuDepartments of Blood Transfusion, Nephrology, Orthopaedic Surgery, and General Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Bo HuangMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Yixiao FengDepartments of Blood Transfusion, Nephrology, Orthopaedic Surgery, and General Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Bo ZhangMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Rex C HaydonMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Hue H LuuMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Russell R ReidMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Michael J LeeMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Jennifer Moriatis WolfMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Zebo YuDepartments of Blood Transfusion, Nephrology, Orthopaedic Surgery, and General Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Tong-Chuan HeMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Funding
VIRAL ONCOLOGY CORE FACILITYP30CA014599 · NCI · UNIVERSITY OF CHICAGO · PI MARK W. LINGEN · 1985 to 2026
$122.1MRe-Engineering Translational Research at the University of ChicagoUL1TR000430 · NCATS · UNIVERSITY OF CHICAGO · PI SOLWAY, JULIAN · 2012 to 2016
$20.2MCenter for Herbal Research on Colorectal CancerP01AT004418 · NCCIH · UNIVERSITY OF CHICAGO · PI YUAN, CHUN-SU · 2008 to 2012
$5.9MDifferential Effects of BMPs on the Healing of Craniofacial DefectsK08DE020140 · NIDCR · UNIVERSITY OF CHICAGO · PI REID, RUSSELL R. · 2009 to 2013
$624kNCATS NIH HHS UL1 TR000430NCCIH NIH HHS P01 AT004418NCI NIH HHS P30 CA014599NIDCR NIH HHS K08 DE020140
Abstract
Mesenchymal stem cells (MSCs) are multipotent non-hematopoietic progenitor cells that can undergo self-renewal and differentiate into multi-lineages. Bone marrow stromal stem cells (BMSCs) represent one of the most commonly-used MSCs. In order to overcome the technical challenge of maintaining primary BMSCs in long-term culture, here we seek to establish reversibly immortalized mouse BMSCs (imBMSCs). By exploiting CRISPR/Cas9-based homology-directed-repair (HDR) mechanism, we target SV40T to mouse
Indexed as
BMP9bone marrow stromal stem cells (BMSCs)CRISPR/Cas9 genome-editingmesenchymal stem cells (MSCs)osteogenic differentiation
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.