ArticlePlant methods2018
A new toolset for protein expression and subcellular localization studies in citrus and its application to
Article in Plant methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 17 citations in OpenAlex.
- Recombinant cystatin ingestion byFrontiers in insect science · 2026Article
- An efficient and broadly applicable method for transient transformation of plants using vertically aligned carbon nanofiber arrays.Frontiers in plant science · 2022Article
- A simple and efficient agroinfiltration method for transient gene expression in Citrus.Plant cell reports · 2021Article
- Molecular signatures of silencing suppression degeneracy from a complex RNA virus.PLoS computational biology · 2021Article
- Article
- Citrus Genetic Transformation: An Overview of the Current Strategies and Insights on the New Emerging Technologies.Frontiers in plant science · 2021Review
- Particle bombardment technology and its applications in plants.Molecular biology reports · 2020Review
- Biolistic Approach for Transient Gene Expression Studies in Plants.Methods in molecular biology (Clifton, N.J.) · 2020Review
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
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Abstract
backgroundTransient gene expression is a powerful tool to study gene function in plants. In citrus,
resultsHere we describe an improved method for gene expression in epidermal cells of citrus leaves, using the Bio-Rad Helios gene-gun. Gene-gun bombardment of GFP-HDEL produced highly efficient gene expression in large number of cells and in different citrus varieties. We show here that transiently expressed proteins have maintained their functions in plants, and this is demonstrated by the subcellular localization of different organelle markers, and by a functional assay of
conclusionWe have optimized a new method for transient expression in citrus leaves, to give highly reproducible and efficient transformation without producing a high level of injury or artifacts to the bombarded tissue. We also generated the first set organelle markers for use in citrus. These fluorescent protein markers label the nucleus and the actin. With these new resources, protein activity and subcellular localization can be studied in citrus rapidly and in high throughput. The handheld gene-gun device can also be used in the grove to deliver therapies for citrus diseases, such as canker and Huanglongbing, into trees.
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