Evidence map›Paper›PMID 29339826›Full record

ArticleScientific reports2018

Using intracellular markers to identify a novel set of surface markers for live cell purification from a heterogeneous hIPSC culture.

Elizabeth J Paik, Alison L O'Neil, Shi-Yan Ng, Chicheng Sun, Lee L Rubin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 21 citations in OpenAlex.

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  8. The quest of cell surface markers for stem cell therapy.Cellular and molecular life sciences : CMLS · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Elizabeth J PaikDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Alison L O'NeilDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Shi-Yan NgDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Chicheng SunDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.
Lee L RubinDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA. lee_rubin@harvard.edu.
Harvard University · USHarvard Stem Cell Institute · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) can provide sources for midbrain dopaminergic (mDA) neural progenitors (NPCs) for cell therapy to treat Parkinson's disease (PD) patients. However, the well-known line-to-cell line variability in the differentiation capacity of individual cell lines needs to be improved for the success of this therapy. To address this issue, we sought to identify mDA NPC specific cell surface markers for fluorescence activated cell sorting (FACS). Through RNA isolation after sorting for NPCs based on staining for cell-specific transcription factors followed by microarray, we identified two positive cell surface markers (CORIN and CD166) and one negative cell surface marker (CXCR4) for mDA NPC sorting. These three markers can enrich floor plate NPCs to 90% purity, and the sorted NPCs more efficiently differentiate to mature dopaminergic neurons compared to unsorted or CORIN

Indexed as

Activated-Leukocyte Cell Adhesion MoleculeAntigens, CDBiomarkersCell Adhesion Molecules, NeuronalFetal ProteinsFlow CytometryHuman Embryonic Stem CellsHumansInduced Pluripotent Stem CellsMembrane ProteinsReceptors, CXCR4Serine EndopeptidasesActivated-Leukocyte Cell Adhesion MoleculeALCAM protein, humanAntigens, CDBiomarkersCell Adhesion Molecules, NeuronalCORIN protein, humanCXCR4 protein, humanFetal ProteinsMembrane ProteinsReceptors, CXCR4Serine Endopeptidases

Identifiers

PMID29339826
PMCPMC5770419
OpenAlexW2782758340

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.