ArticleScientific reports2018
Development and optimization of a differentiated airway epithelial cell model of the bovine respiratory tract.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- Animal organoids as models for integrated One Health research.One health (Amsterdam, Netherlands) · 2026Review
- Characterizing airway epithelial cultures grown on permeable inserts with different pore densities.Physiological reports · 2026Article
- The Development of 3D Primary Co-Culture Models of the Human Airway.International journal of molecular sciences · 2025Article
- Primary cell culture systems to investigate host-pathogen interactions in bacterial respiratory tract infections of livestock.Frontiers in cellular and infection microbiology · 2025Review
- The Pestivirus RNase EViruses · 2024Article
- In Vitro Characteristics of Canine Primary Tracheal Epithelial Cells Maintained at an Air-Liquid Interface Compared to In Vivo Morphology.International journal of molecular sciences · 2023Article
- Development of human-derived, three-dimensional respiratory epithelial tissue constructs with perfusable microvasculature on a high-throughput microfluidics screening platform.Biofabrication · 2022Article
- Bovine respiratory microbiota of feedlot cattle and its association with disease.Veterinary research · 2022Review
- Development and evaluation of a bovine lung-on-chip (bLOC) to study bovine respiratory diseases.In vitro models · 2022Article
- Bone Marrow Derived Mesenchymal Stromal Cells Promote Vascularization and Ciliation in Airway Mucosa Tri-Culture ModelsFrontiers in bioengineering and biotechnology · 2022Article
- Isolation and development of bovine primary respiratory cells as model to study influenza D virus infection.Virology · 2021Article
- State of the art on lung organoids in mammals.Veterinary research · 2021Review
- The specific features of the developing T cell compartment of the neonatal lung are a determinant of respiratory syncytial virus immunopathogenesis.PLoS pathogens · 2021Article
- Differentiated ovine tracheal epithelial cells support the colonisation of pathogenic and non-pathogenic strains of Mannheimia haemolytica.Scientific reports · 2020Article
- Mannheimia haemolytica and lipopolysaccharide induce airway epithelial inflammatory responses in an extensively developed ex vivo calf model.Scientific reports · 2020Article
- Choosing the Right Differentiation Medium to Develop Mucociliary Phenotype of Primary Nasal Epithelial Cells In Vitro.Scientific reports · 2020Article
- Pathogenic Mannheimia haemolytica Invades Differentiated Bovine Airway Epithelial Cells.Infection and immunity · 2019Article
- Non-woven bilayered biodegradable chitosan-gelatin-polylactide scaffold for bioengineering of tracheal epithelium.Cell proliferation · 2019Article
- Rock Inhibitor Y-27632 Enables Feeder-Free, Unlimited Expansion ofStem cells international · 2019Article
- Temporal differentiation of bovine airway epithelial cells grown at an air-liquid interface.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Cattle are subject to economically-important respiratory tract infections by various bacterial and viral pathogens and there is an urgent need for the development of more realistic in vitro models of the bovine respiratory tract to improve our knowledge of disease pathogenesis. In the present study, we have optimized the culture conditions in serum-free medium that allow bovine bronchial epithelial cells (BBECs) grown at an air-liquid interface to differentiate into a three-dimensional epithelium that is highly representative of the bovine airway. Epidermal growth factor was required to trigger both proliferation and differentiation of BBECs whilst retinoic acid was also essential for mucociliary differentiation. Triiodothyronine was demonstrated not to be important for the differentiation of BBECs. Oxygen concentration had a minimal effect although optimal ciliation was achieved when BBECs were cultured at 14% oxygen tension. Insert pore-density had a significant effect on the growth and differentiation of BBECs; a high-pore-density was required to trigger optimum differentiation. The established BBEC model will have wide-ranging applications for the study of bacterial and viral infections of the bovine respiratory tract; it will contribute to the development of improved vaccines and therapeutics and will reduce the use of cattle in in vivo experimentation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.