Evidence map›Paper›PMID 29339418›Full record

ArticleJournal of bacteriology2018

An Evolutionary/Biochemical Connection between Promoter- and Primer-Dependent Polymerases Revealed by Systematic Evolution of Ligands by Exponential Enrichment.

Katherine J Fenstermacher, Vasudevan Achuthan, Thomas D Schneider, Jeffrey J DeStefano

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of bacteriology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Katherine J FenstermacherCell Biology and Molecular Genetics, University of Maryland, College Park, Maryland, USA.ORCID 0000-0003-1139-3711
Vasudevan AchuthanCell Biology and Molecular Genetics, University of Maryland, College Park, Maryland, USA.
Thomas D SchneiderNational Institutes of Health, National Cancer Institute, Center for Cancer Research, RNA Biology Laboratory, Frederick, Maryland, USA.
Jeffrey J DeStefanoCell Biology and Molecular Genetics, University of Maryland, College Park, Maryland, USA jdestefa@umd.edu.ORCID 0000-0002-8710-7622
University of Maryland, College Park · USNational Institutes of Health · US

Funding

Biochemistry of HIV reverse transcriptase fidelity and inhibitor interactionsR01GM116645 · NIGMS · UNIV OF MARYLAND, COLLEGE PARK · PI DESTEFANO, JEFFREY J · 2016 to 2018
$924k
Biochemistry of HIV reverse transcriptase fidelity and inhibitor interactionsR01AI150480 · NIAID · UNIV OF MARYLAND, COLLEGE PARK · PI DESTEFANO, JEFFREY J · 2019 to 2019
$294k
NIAID NIH HHS R01 AI150480NIGMS NIH HHS R01 GM116645
6 · The paper itself

Abstract

DNA polymerases (DNAPs) recognize 3' recessed termini on duplex DNA and carry out nucleotide catalysis. Unlike promoter-specific RNA polymerases (RNAPs), no sequence specificity is required for binding or initiation of catalysis. Despite this, previous results indicate that viral reverse transcriptases bind much more tightly to DNA primers that mimic the polypurine tract. In the current report, primer sequences that bind with high affinity to

Indexed as

Promoter Regions, GeneticDNA, BacterialDNA-Directed DNA PolymeraseDNA-Directed RNA PolymerasesEscherichia coliEvolution, MolecularKineticsLigandsPolymerase Chain ReactionSELEX Aptamer TechniqueSubstrate SpecificityViral Proteinsbacteriophage T7 RNA polymeraseDNA, BacterialDNA-Directed DNA PolymeraseDNA-Directed RNA PolymerasesLigandsViral ProteinsaptamerDNA polymerasemolecular evolutionmultiplex PCRPCR primer biasRNA polymerasesSELEXT3 RNA polymeraseT7 RNA polymerase

Identifiers

PMID29339418
PMCPMC5847653
OpenAlexW2784177800

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.