ArticleJournal of molecular endocrinology2018
Intact glucose uptake despite deteriorating signaling in adipocytes with high-fat feeding.
Article in Journal of molecular endocrinology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Beneficial effect on serum cholesterol levels, but not glycaemic regulation, after replacing SFA with PUFA for 3 d: a randomised crossover trial.The British journal of nutrition · 2021Trial
- Article
- Adipocyte traits limiting cellular insulin responsiveness and glucose transport.American journal of physiology. Endocrinology and metabolism · 2023Article
- EHD2 regulates plasma membrane integrity and downstream insulin receptor signaling events.Molecular biology of the cell · 2023Article
- Estradiol cycling drives female obesogenic adipocyte hyperplasia.Cell reports · 2023Article
- Integrated experimental-computational analysis of a HepaRG liver-islet microphysiological system for human-centric diabetes research.PLoS computational biology · 2022Article
- A compound directed against S6K1 hampers fat mass expansion and mitigates diet-induced hepatosteatosis.JCI insight · 2022Article
- Estradiol replacement improves high-fat diet-induced insulin resistance in ovariectomized rats.Physiological reports · 2022Article
- Expansion of the Inguinal Adipose Tissue Depot Correlates With Systemic Insulin Resistance in C57BL/6J Mice.Frontiers in cell and developmental biology · 2022Article
- EH Domain-Containing 2 Deficiency Restricts Adipose Tissue Expansion and Impairs Lipolysis in Primary Inguinal Adipocytes.Frontiers in physiology · 2021Article
- Adipose Morphology: a Critical Factor in Regulation of Human Metabolic Diseases and Adipose Tissue Dysfunction.Obesity surgery · 2020Review
- Inner ear is a target for insulin signaling and insulin resistance: evidence from mice and auditory HEI-OC1 cells.BMJ open diabetes research & care · 2020Article
- Adipose cell size changes are associated with a drastic actin remodeling.Scientific reports · 2019Article
- EHD2 regulates adipocyte function and is enriched at cell surface-associated lipid droplets in primary human adipocytes.Molecular biology of the cell · 2019Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
To capture immediate cellular changes during diet-induced expansion of adipocyte cell volume and number, we characterized mature adipocytes during a short-term high-fat diet (HFD) intervention. Male C57BL6/J mice were fed chow diet, and then switched to HFD for 2, 4, 6 or 14 days. Systemic glucose clearance was assessed by glucose tolerance test. Adipose tissue was dissected for RNA-seq and cell size distribution analysis using coulter counting. Insulin response in isolated adipocytes was monitored by glucose uptake assay and Western blotting, and confocal microscopy was used to assess autophagic activity. Switching to HFD was accompanied by an immediate adipocyte size expansion and onset of systemic insulin resistance already after two days, followed by recruitment of new adipocytes. Despite an initially increased non-stimulated and preserved insulin-stimulated glucose uptake, we observed a decreased phosphorylation of insulin receptor substrate-1 (IRS-1) and protein kinase B (PKB). After 14 days of HFD, both the insulin-stimulated phosphorylation of Akt substrate of 160 kDa (AS160) and glucose uptake was blunted. RNA-seq analysis of adipose tissue revealed transient changes in gene expression at day four, including highly significant upregulation of
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.