ArticleAnalytical biochemistry2018
Moving toward rapid and low-cost point-of-care molecular diagnostics with a repurposed 3D printer and RPA.
Article in Analytical biochemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A need to raise the bar - A systematic review of temporal trends in diagnostics for Japanese encephalitis virus infection, and perspectives for future research.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2020Pooled it
- Trends of nucleic acid - based point-of-care diagnostics for infectious diseases.Journal of biological engineering · 2026Review
- A low-cost color sensor device for rapid detection of high-grade serous ovarian carcinoma (HGSOC).Oncoscience · 2026Article
- Integrating RPA-LFD and TaqMan qPCR for Rapid On-Site Screening and Accurate Laboratory Identification ofFoods (Basel, Switzerland) · 2025Article
- Development of a Colorimetric Loop-Mediated Isothermal Amplification Assay for the Detection ofDiagnostics (Basel, Switzerland) · 2024Article
- The Development of a 3D Printer-Inspired, Microgravity-Compatible Sample Preparation Device for Future Use Inside the International Space Station.Micromachines · 2023Article
- Loop-Mediated Isothermal Amplification ofMicroorganisms · 2022Article
- Parasitological, serological and molecular diagnosis of acute and chronic Chagas disease: from field to laboratory.Memorias do Instituto Oswaldo Cruz · 2022Article
- Comparison of Design Approaches for Low-Cost Sampling Mechanisms in Open-Source Chemical Instrumentation.HardwareX · 2021Article
- Narrative review of gene modification: applications in three-dimensional (3D) bioprinting.Annals of translational medicine · 2021Review
- 3D Printed Bioconstructs: Regenerative Modulation for Genetic Expression.Stem cell reviews and reports · 2021Review
- Low-cost and open-source strategies for chemical separations.Journal of chromatography. A · 2021Review
- PrintrLab incubator: A portable and low-cost CO2 incubator based on an open-source 3D printer architecture.PloS one · 2021Article
- Developments in integrating nucleic acid isothermal amplification and detection systems for point-of-care diagnostics.Biosensors & bioelectronics · 2020Review
- Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases.Biosensors & bioelectronics · 2020Review
- A low-cost smart system for electrophoresis-based nucleic acids detection at the visible spectrum.PloS one · 2020Article
- Diagnostics in space: will zero gravity add weight to new advances?Expert review of molecular diagnostics · 2020Article
- Interfacing Pathogen Detection with Smartphones for Point-of-Care Applications.Analytical chemistry · 2019Review
- Advances in Diagnostic Methods for Zika Virus Infection.Journal of medical devices · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Traditionally, the majority of nucleic acid amplification-based molecular diagnostic tests are done in centralized settings. In recent years, point-of-care tests have been developed for use in low-resource settings away from central laboratories. While most experts agree that point-of-care molecular tests are greatly needed, their availability as cost-effective and easy-to-operate tests remains an unmet goal. In this article, we discuss our efforts to develop a recombinase polymerase amplification reaction-based test that will meet these criteria. First, we describe our efforts in repurposing a low-cost 3D printer as a platform that can carry out medium-throughput, rapid, and high-performing nucleic acid extraction. Next, we address how these purified templates can be rapidly amplified and analyzed using the 3D printer's heated bed or the deconstructed, low-cost thermal cycler we have developed. In both approaches, real-time isothermal amplification and detection of template DNA or RNA can be accomplished using a low-cost portable detector or smartphone camera. Last, we demonstrate the capability of our technologies using foodborne pathogens and the Zika virus. Our low-cost approach does not employ complicated and high-cost components, making it suitable for resource-limited settings. When integrated and commercialized, it will offer simple sample-to-answer molecular diagnostics.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.