ArticleBMC biology2018
A live cell assay of GPCR coupling allows identification of optogenetic tools for controlling Go and Gi signaling.
Article in BMC biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 59 citations in OpenAlex.
- Principles of ribosome-associated protein quality control during the synthesis of CFTR.The EMBO journal · 2026Article
- Functional methods for evaluating the efficacy of retinal optogenetic therapy for vision restoration.Frontiers in neuroscience · 2026Review
- Differential increase in endocannabinoid levels at the plasma and intracellular membranes.iScience · 2025Article
- GNature communications · 2025Article
- Molecular basis underlying the specificity of an antagonist AA92593 for mammalian melanopsins.The Journal of biological chemistry · 2025Article
- Transcriptomic analysis reveals a critical role for activating GScientific reports · 2024Article
- Gain control of sensory input across polysynaptic circuitries in mouse visual cortex by a single G protein-coupled receptor type (5-HTNature communications · 2024Article
- Ectopically expressed rhodopsin is not sensitive to X-rays.BMC neuroscience · 2024Article
- Gz Enhanced Signal Transduction assaY (GbioRxiv : the preprint server for biology · 2024Article
- A bistable inhibitory optoGPCR for multiplexed optogenetic control of neural circuits.Nature methods · 2024Article
- Optimized design and in vivo application of optogenetically functionalized Drosophila dopamine receptors.Nature communications · 2023Article
- Modulating signalling lifetime to optimise a prototypical animal opsin for optogenetic applications.Pflugers Archiv : European journal of physiology · 2023Article
- Highly sensitive visual restoration and protection via ectopic expression of chimeric rhodopsin in mice.iScience · 2023Article
- A bistable inhibitory OptoGPCR for multiplexed optogenetic control of neural circuits.bioRxiv : the preprint server for biology · 2023Article
- A self-inactivating invertebrate opsin optically drives biased signaling toward Gβγ-dependent ion channel modulation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Functional optimization of light-activatable Opto-GPCRs: Illuminating the importance of the proximal C-terminus in G-protein specificity.Frontiers in cell and developmental biology · 2023Article
- Chimeric human opsins as optogenetic light sensitisers.The Journal of experimental biology · 2021Article
- Decoding LncRNAs.Cancers · 2021Review
- Article
- Seasonal variation in UVA light drives hormonal and behavioural changes in a marine annelid via a ciliary opsin.Nature ecology & evolution · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAnimal opsins are light-sensitive G-protein-coupled receptors (GPCRs) that enable optogenetic control over the major heterotrimeric G-protein signaling pathways in animal cells. As such, opsins have potential applications in both biomedical research and therapy. Selecting the opsin with the best balance of activity and selectivity for a given application requires knowing their ability to couple to a full range of relevant Gα subunits. We present the GsX assay, a set of tools based on chimeric Gs subunits that transduce coupling of opsins to diverse G proteins into increases in cAMP levels, measured with a real-time reporter in living cells. We use this assay to compare coupling to Gi/o/t across a panel of natural and chimeric opsins selected for potential application in gene therapy for retinal degeneration.
resultsOf the opsins tested, wild-type human rod opsin had the highest activity for chimeric Gs proxies for Gi and Gt (Gsi and Gst) and was matched in Go proxy (Gso) activity only by a human rod opsin/scallop opsin chimera. Rod opsin drove roughly equivalent responses via Gsi, Gso, and Gst, while cone opsins showed much lower activities with Gso than Gsi or Gst, and a human rod opsin/amphioxus opsin chimera demonstrated higher activity with Gso than with Gsi or Gst. We failed to detect activity for opsin chimeras bearing three intracellular fragments of mGluR6, and observed unexpectedly complex response profiles for scallop and amphioxus opsins thought to be specialized for Go.
conclusionsThese results identify rod opsin as the most potent non-selective Gi/o/t-coupled opsin, long-wave sensitive cone opsin as the best for selectively activating Gi/t over Go, and a rod opsin/amphioxus opsin chimera as the best choice for selectively activating Go over Gi/t.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.