ArticlePloS one2018
Quantitative in vivo mapping of myocardial mitochondrial membrane potential.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Validation of Cardiorespiratory-Gated Proton Beam Delivery for Stereotactic Arrhythmia Radioablation.Journal of cardiovascular electrophysiology · 2026Article
- Sarcolemmal and mitochondrial membrane potentials measured ex vivo and in vivo in the heart by pharmacokinetic modelling of [The Journal of physiology · 2026Article
- Rapid mitochondrial repolarization upon reperfusion after cardiac ischemia.Nature cardiovascular research · 2025Article
- Controlling Electron Flow in Carbofluorescein Voltage Indicators.ACS chemical biology · 2025Article
- In vivo 3D myocardial membrane potential mapping in humans using PET/MRI.EJNMMI research · 2025Article
- How and when to measure mitochondrial inner membrane potentials.Biophysical journal · 2024Review
- Tracers for Cardiac Imaging: Targeting the Future of Viable Myocardium.Pharmaceutics · 2023Review
- From mitochondria to cells to humans: Targeting bioenergetics in aging and disease.The international journal of biochemistry & cell biology · 2023Article
- Mitochondrial Damage Induced by T-2 Mycotoxin on Human Skin-Fibroblast Hs68 Cell Line.Molecules (Basel, Switzerland) · 2023Article
- Common methods in mitochondrial research (Review).International journal of molecular medicine · 2022Article
- Flipping the Switch: Reverse-Demand Voltage-Sensitive Fluorophores.Journal of the American Chemical Society · 2022Article
- PET imaging of mitochondrial function in acute doxorubicin-induced cardiotoxicity: a proof-of-principle study.Scientific reports · 2022Article
- Anthracycline-induced cardiotoxicity: From pathobiology to identification of molecular targets for nuclear imaging.Frontiers in cardiovascular medicine · 2022Review
- Cardiac hybrid imaging: novel tracers for novel targets.Journal of geriatric cardiology : JGC · 2021Article
- Cardio-Oncology: Understanding the Intersections Between Cardiac Metabolism and Cancer Biology.JACC. Basic to translational science · 2021Review
- Mitochondrial energetics with transmembrane electrostatically localized protons: do we have a thermotrophic feature?Scientific reports · 2021Article
- Energy Renewal: Isothermal Utilization of Environmental Heat Energy with Asymmetric Structures.Entropy (Basel, Switzerland) · 2021Article
- Quantification of Myocardial Mitochondrial Membrane Potential Using PET.Current cardiology reports · 2021Review
- Quantitative PET in the 2020s: a roadmap.Physics in medicine and biology · 2021Article
- In vivo quantitative mapping of human mitochondrial cardiac membrane potential: a feasibility study.European journal of nuclear medicine and molecular imaging · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
13 authors.
Funding
Abstract
backgroundMitochondrial membrane potential (ΔΨm) arises from normal function of the electron transport chain. Maintenance of ΔΨm within a narrow range is essential for mitochondrial function. Methods for in vivo measurement of ΔΨm do not exist. We use 18F-labeled tetraphenylphosphonium (18F-TPP+) to measure and map the total membrane potential, ΔΨT, as the sum of ΔΨm and cellular (ΔΨc) electrical potentials.
methodsEight pigs, five controls and three with a scar-like injury, were studied. Pigs were studied with a dynamic PET scanning protocol to measure 18F-TPP+ volume of distribution, VT. Fractional extracellular space (fECS) was measured in 3 pigs. We derived equations expressing ΔΨT as a function of VT and the volume-fractions of mitochondria and fECS. Seventeen segment polar maps and parametric images of ΔΨT were calculated in millivolts (mV).
resultsIn controls, mean segmental ΔΨT = -129.4±1.4 mV (SEM). In pigs with segmental tissue injury, ΔΨT was clearly separated from control segments but variable, in the range -100 to 0 mV. The quality of ΔΨT maps was excellent, with low noise and good resolution. Measurements of ΔΨT in the left ventricle of pigs agree with previous in in-vitro measurements.
conclusionsWe have analyzed the factors affecting the uptake of voltage sensing tracers and developed a minimally invasive method for mapping ΔΨT in left ventricular myocardium of pigs. ΔΨT is computed in absolute units, allowing for visual and statistical comparison of individual values with normative data. These studies demonstrate the first in vivo application of quantitative mapping of total tissue membrane potential, ΔΨT.
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