ArticlePLoS genetics2018
Wor1 establishes opaque cell fate through inhibition of the general co-repressor Tup1 in Candida albicans.
Article in PLoS genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 30 citations in OpenAlex.
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- The Gti1/Pac2 family protein CFG1 controls fungal chlamydospore formation through orchestrating cell wall remodeling, lipid metabolism, and ribosome biogenesis.Applied and environmental microbiology · 2026Article
- The transcriptional repressor Ssn6 modulates phase separation to regulate fungal gene expression.mBio · 2026Article
- Genetic and environmental control of parasexual reproduction in the pathogenicMicrobiology and molecular biology reviews : MMBR · 2025Review
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- Gene dosage and protein valency impact phase separation and fungal cell fate.PLoS genetics · 2025Article
- White-opaque switching inMicrobiology and molecular biology reviews : MMBR · 2024Review
- Transcriptional regulation of the synthesis and secretion of farnesol in the fungus Candida albicans: examination of the Homann transcription regulator knockout collection.G3 (Bethesda, Md.) · 2023Article
- Integrated analysis of SR-like protein kinases Sky1 and Sky2 links signaling networks with transcriptional regulation inFrontiers in cellular and infection microbiology · 2023Article
- Fluorescent toys 'n' tools lighting the way in fungal research.FEMS microbiology reviews · 2021Article
- BbWor1, a Regulator of Morphological Transition, Is Involved in Conidium-Hypha Switching, Blastospore Propagation, and Virulence in Beauveria bassiana.Microbiology spectrum · 2021Article
- The Roles of Chromatin Accessibility in Regulating theJournal of fungi (Basel, Switzerland) · 2021Review
- Parasexuality ofFrontiers in cellular and infection microbiology · 2021Review
- Mechanism of Candida pathogenesis: revisiting the vital drivers.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Review
- Candida albicans Double Mutants Lacking bothmSphere · 2020Article
- Coordinate genomic association of transcription factors controlled by an imported quorum sensing peptide in Cryptococcus neoformans.PLoS genetics · 2020Article
- Proteomic profiling of the monothiol glutaredoxin Grx3 reveals its global role in the regulation of iron dependent processes.PLoS genetics · 2020Article
- Monitoring Phenotypic Switching in Candida albicans and the Use of Next-Gen Fluorescence Reporters.Current protocols in microbiology · 2019Article
- Attack, Defend and Persist: How the Fungal Pathogen Candida auris was Able to Emerge Globally in Healthcare Environments.Mycopathologia · 2019Review
- Roles of the Transcription Factors Sfl2 and Efg1 in White-Opaque Switching in a/α Strains of Candida albicans.mSphere · 2019Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
The pathogenic fungus Candida albicans can undergo phenotypic switching between two heritable states: white and opaque. This phenotypic plasticity facilitates its colonization in distinct host niches. The master regulator WOR1 is exclusively expressed in opaque phase cells. Positive feedback regulation by Wor1 on the WOR1 promoter is essential for opaque formation, however the underlying mechanism of how Wor1 functions is not clear. Here, we use tandem affinity purification coupled with mass spectrometry to identify Wor1-interacting proteins. Tup1 and its associated complex proteins are found as the major factors associated with Wor1. Tup1 occupies the same regions of the WOR1 promoter as Wor1 preferentially in opaque cells. Loss of Tup1 is sufficient to induce the opaque phase, even in the absence of Wor1. This is the first such report of a bypass of Wor1 in opaque formation. These genetic analyses suggest that Tup1 is a key repressor of the opaque state, and Wor1 functions via alleviating Tup1 repression at the WOR1 promoter. Opaque cells convert to white en masse at 37°C. We show that this conversion occurs only in the presence of glycolytic carbon sources. The opaque state is stabilized when cells are cultured on non-glycolytic carbon sources, even in a MTLa/α background. We further show that temperature and carbon source affect opaque stability by altering the levels of Wor1 and Tup1 at the WOR1 promoter. We propose that Wor1 and Tup1 form the core regulatory circuit controlling the opaque transcriptional program. This model provides molecular insights on how C. albicans adapts to different host signals to undergo phenotypic switching for colonization in distinct host niches.
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