Evidence map›Paper›PMID 29336475›Full record

ArticleJournal of cellular physiology2018

Exosomes originating from MSCs stimulated with TGF-β and IFN-γ promote Treg differentiation.

Qingyi Zhang, Lin Fu, Yahui Liang, Zikuan Guo, Lisheng Wang, Cong Ma, Hengxiang Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular physiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 120 papers.

0numbers the graph read from it
0cells of the map it votes in
120citing papers in PubMed
7.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

120 citing papers in PubMed, 180 citations in OpenAlex.

  1. Review
  2. Review
  3. METTL3-mCellular & molecular biology letters · 2026
    Article
  4. Article
  5. Review
  6. Review
  7. From pathogenesis to therapy: extracellular vesicles in osteoarthritis.Frontiers in bioengineering and biotechnology · 2026
    Review
  8. Review
  9. Review
  10. Review
  11. Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Exosomes and Exosome-Mimetics for Atopic Dermatitis Therapy.Tissue engineering and regenerative medicine · 2025
    Review
  17. Microenvironmental Modulation for Therapeutic Efficacy of Extracellular Vesicles.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Review
  18. Article
  19. Review
  20. Review

60 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Qingyi ZhangDepartment of Hematology of Air Force PLA General Hospital, Beijing, China.ORCID http://orcid.org/0000-0002-2328-1453
Lin FuDepartment of Hematology and Lymphoma Research Center, Peking University, Third Hospital, Beijing, China.
Yahui LiangDepartment of Hematology of Air Force PLA General Hospital, Beijing, China.
Zikuan GuoMilitary Medical Science Academy of the PLA Institute of Radiation Medicine, Beijing, China.
Lisheng WangMilitary Medical Science Academy of the PLA Institute of Radiation Medicine, Beijing, China.
Cong MaDepartment of Hematology of Air Force PLA General Hospital, Beijing, China.
Hengxiang WangDepartment of Hematology of Air Force PLA General Hospital, Beijing, China.
Air Force General Hospital PLA · CNAcademy of Military Medical Sciences · CNFirst Affiliated Hospital of Xinxiang Medical University · CNXuzhou Medical College · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mesenchymal stem cells (MSCs) have been approved as a cellular drug for the treatment of a variety of immune-related diseases by the government of many countries'. Previous investigations, including ours, have shown that exosomes secreted by MSCs (MSC-ex) are one of the main factors responsible for the therapeutic effect of MSCs. However, the immune modulation activities and the contents of MSC-ex derived from cells under different incubation conditions differ dramatically. Therefore, the optimal way to ensure effectiveness is by identifying and preparing MSC-ex with confirmed potent immunosuppressive activity. The aim of this study was to investigate and analyze the composition and function of MSC-ex secreted by MSCs stimulated by different cytokines to obtain exosomes with more potent immunosuppressive activity. To achieve this aim, umbilical cord-derived MSCs were treated with PBS, TGF-β, IFN-γ, or TGF-β plus IFN-γ for 72 hr. Then, exosomes were isolated from the culture supernatants. Common exosome markers, such as CD9, CD63, and CD81, were detected and analyzed by FCM. At the same time, the TGF-β, IFN-γ, IDO, and IL-10 content in exosomes was detected, and the influence of exosmes from defferent groups on the induction of mononuclear cell transformation into Tregs was analyzed via FCM. Our results show that the TGF-β combined with IFN-γ exosome group more effectively promoted the transformation of mononuclear cells to Tregs, and the analysis showed that IDO may play an important role. This study might provide a novel strategy to treat GVHD as well as other immune-associated disorders.

Indexed as

Cell DifferentiationCells, CulturedCytokinesExosomesHumansImmunomodulationInterferon-gammaInterleukin-10Leukocytes, MononuclearLymphocyte ActivationMesenchymal Stem CellsT-Lymphocytes, RegulatoryTransforming Growth Factor betaUmbilical CordCytokinesInterferon-gammaInterleukin-10Transforming Growth Factor betacytokineexosomeIFN-γmesenchymal stem cellsTGF-β

Identifiers

PMID29336475
PMCPMC13482522
OpenAlexW2807572737

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.